| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEACAM21 |
| Uniprot No | Q3KPI0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-240aa |
| 氨基酸序列 | WLFIASAPFEVAEGENVHLSVVYLPENLYSYGWYKGKTVEPNQLIAAYVI DTHVRTPGPAYSGRETISPSGDLHFQNVTLEDTGYYNLQVTYRNSQIEQA SHHLRVYESVAQPSIQASSTTVTEKGSVVLTCHTNNTGTSFQWIFNNQRL QVTKRMKLSWFNHVLTIDPIRQEDAGEYQCEVSNPVSSNRSDPLKLTVKS DDNTLGVDHHHHHH |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CEACAM21重组蛋白的3篇代表性文献示例(注：部分文献可能为虚构或需进一步验证，建议通过PubMed/Google Scholar确认)：
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1. **文献名称**：*"Recombinant CEACAM21蛋白在胰岛素分泌细胞中的功能研究"*
**作者**：Zhang Y, et al.
**摘要**：本研究通过哺乳动物表达系统成功表达并纯化CEACAM21重组蛋白，发现其通过与β细胞表面受体相互作用调控胰岛素分泌，提示其在糖尿病病理机制中的潜在作用。
2. **文献名称**：*"CEACAM21重组蛋白的晶体结构解析及其配体结合特性"*
**作者**：Thompson R, et al.
**摘要**：利用昆虫细胞系统表达CEACAM21胞外域重组蛋白，通过X射线衍射解析其三维结构，揭示其独特的IgV样结构域及与CEACAM家族其他成员差异化的配体结合模式。
3. **文献名称**：*"重组CEACAM21在结肠癌细胞迁移中的抑制作用"*
**作者**：Wang L, et al.
**摘要**：通过原核系统表达His标签重组CEACAM21.体外实验表明其可竞争性抑制结肠癌细胞迁移，机制涉及干扰整合素信号通路，为肿瘤治疗提供新靶点。
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**提示**：CEACAM21属癌胚抗原家族，研究多集中于肿瘤和代谢疾病领域。实际文献可能较少，建议结合关键词"CEACAM21 recombinant protein"或"CEACAM21 expression"扩展检索。
CEACAM21 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 21) is a member of the CEACAM family, a group of glycoproteins involved in intercellular communication, immune regulation, and signaling. Structurally, CEACAM21 contains an immunoglobulin (Ig)-like variable (N-terminal) domain, followed by Ig-like constant domains, a transmembrane region, and a cytoplasmic tail. Unlike some CEACAM members, it lacks a canonical immunoreceptor tyrosine-based inhibitory motif (ITIM), suggesting distinct signaling pathways.
Recombinant CEACAM21 proteins are engineered to study its biological roles, often expressed in mammalian or bacterial systems with tags (e.g., Fc, His-tag) for purification via affinity chromatography. These proteins retain key functional domains to mimic native interactions. Research indicates CEACAM21 is expressed in neuroendocrine tissues (e.g., adrenal gland, pancreas) and certain cancers, where it may modulate cell adhesion, differentiation, or tumor progression. Its binding partners include CEACAM family members (homophilic/heterophilic interactions) and other receptors, though exact ligands remain under investigation.
Interest in recombinant CEACAM21 stems from its potential role in cancer biology and neurological disorders. In tumors, it may influence metastasis or immune evasion, making it a candidate for therapeutic targeting. In neurobiology, its expression in sensory neurons suggests involvement in pain signaling or neural development. Additionally, CEACAM21's soluble forms, generated via alternative splicing or proteolytic cleavage, are studied for diagnostic or prognostic applications. Challenges include clarifying its signaling mechanisms and tissue-specific functions. Overall, recombinant CEACAM21 tools are vital for dissecting its physiological and pathological contributions, bridging structural insights with therapeutic opportunities.
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