| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALD |
| Uniprot No | Q05682 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-793aa |
| 氨基酸序列 | MDDFERRREL RRQKREEMRL EAERIAYQRN DDDEEEAARE RRRRARQERL RQKQEEESLG QVTDQVEVNA QNSVPDEEAK TTTTNTQVEG DDEAAFLERL ARREERRQKR LQEALERQKE FDPTITDASL SLPSRRMQND TAENETTEKE EKSESRQERY EIEETETVTK SYQKNDWRDA EENKKEDKEK EEEEEEKPKR GSIGENQVEV MVEEKTTESQ EETVVMSLKN GQISSEEPKQ EEEREQGSDE ISHHEKMEEE DKERAEAERA RLEAEERERI KAEQDKKIAD ERARIEAEEK AAAQERERRE AEERERMREE EKRAAEERQR IKEEEKRAAE ERQRIKEEEK RAAEERQRIK EEEKRAAEER QRARAEEEEK AKVEEQKRNK QLEEKKHAMQ ETKIKGEKVE QKIEGKWVNE KKAQEDKLQT AVLKKQGEEK GTKVQAKREK LQEDKPTFKK EEIKDEKIKK DKEPKEEVKS FMDRKKGFTE VKSQNGEFMT HKLKHTENTF SRPGGRASVD TKEAEGAPQV EAGKRLEELR RRRGETESEE FEKLKQKQQE AALELEELKK KREERRKVLE EEEQRRKQEE ADRKLREEEE KRRLKEEIER RRAEAAEKRQ KMPEDGLSDD KKPFKCFTPK GSSLKIEERA EFLNKSVQKS SGVKSTHQAA IVSKIDSRLE QYTSAIEGTK SAKPTKPAAS DLPVPAEGVR NIKSMWEKGN VFSSPTAAGT PNKETAGLKV GVSSRINEWL TKTPDGNKSP APKPSDLRPG DVSSKRNLWE KQSVDKVTSP TKV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CALD(假设指某种钙依赖性蛋白或特定重组蛋白)的示例参考文献及摘要概括，供参考(注：文献为虚构示例，实际需通过学术数据库检索)：
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1. **文献名称**: *Optimized Expression of CALD Recombinant Protein in E. coli for Functional Studies*
**作者**: Zhang, L. et al.
**摘要**: 研究通过大肠杆菌表达系统优化CALD重组蛋白的产量，采用诱导温度调控和密码子优化策略，成功获得高纯度蛋白并验证其钙离子结合活性。
2. **文献名称**: *Structural Characterization of CALD Protein Using Cryo-EM*
**作者**: Patel, R. & Kim, S.
**摘要**: 通过冷冻电镜解析CALD重组蛋白的三维结构，揭示其钙依赖性构象变化机制，为靶向药物设计提供结构基础。
3. **文献名称**: *CALD Recombinant Protein in Neurodegenerative Disease Therapy*
**作者**: Müller, T. et al.
**摘要**: 探讨CALD重组蛋白在阿尔茨海默病模型中的潜在治疗作用，实验显示其可减少神经元钙超载并改善认知功能。
4. **文献名称**: *Comparison of Mammalian vs. Insect Cell Systems for CALD Production*
**作者**: Gupta, A. & Lee, J.
**摘要**: 对比哺乳动物细胞和昆虫细胞表达CALD重组蛋白的优缺点，发现哺乳动物系统表达的蛋白糖基化更完整，活性提高30%。
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**提示**：实际研究中建议在PubMed、Web of Science等平台以关键词“CALD recombinant protein expression”或结合具体研究领域检索最新文献。
**Background of CALD Recombinant Proteins**
CALD (Calcium-dependent Adhesion-Linked Protein) recombinant proteins are engineered molecules designed to study cellular processes involving calcium-mediated adhesion and signaling. Originating from research on cell adhesion mechanisms, CALD proteins are implicated in regulating interactions between cells and the extracellular matrix (ECM), particularly in tissues where calcium ions (Ca²⁺) play a critical role, such as bone, vasculature, and neural systems. These proteins often contain domains that bind calcium, enabling them to modulate cell adhesion, migration, and intracellular signaling pathways.
The development of recombinant CALD proteins leverages genetic engineering to produce purified, functional versions of these proteins in heterologous systems like *E. coli* or mammalian cell cultures. This approach ensures high yield, consistency, and reduced batch-to-batch variability compared to native protein extraction. Recombinant technology also allows for tagging (e.g., His-tag, GFP) to facilitate detection, purification, or tracking in experimental settings.
Applications span basic research and therapeutics. In vitro, CALD recombinant proteins help dissect calcium-dependent adhesion mechanisms or screen drugs targeting related pathways. In regenerative medicine, they may aid in designing biomaterials that enhance tissue repair by mimicking calcium-rich microenvironments. Challenges include maintaining post-translational modifications (e.g., phosphorylation) critical for function, often addressed using mammalian expression systems.
Overall, CALD recombinant proteins serve as versatile tools to explore calcium’s role in health and disease, bridging structural biology and translational innovation. Ongoing advances in protein engineering aim to optimize their stability, specificity, and therapeutic potential.
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