| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALPRO |
| Uniprot No | P05109 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组CALPRO蛋白(Calprotectin)的文献摘要信息，供参考：
1. **文献名称**：Recombinant expression and functional characterization of human calprotectin
**作者**：Damgaard, C., et al.
**摘要**：研究报道了在大肠杆菌中成功表达重组人钙卫蛋白(S100A8/A9复合物)，并验证其金属离子螯合能力及抗菌活性，证实重组蛋白具有与天然蛋白相似的生物功能。
2. **文献名称**：Production of biologically active recombinant human calprotectin in Pichia pastoris
**作者**：Nakashige, T.G., et al.
**摘要**：通过毕赤酵母表达系统高效分泌表达重组人钙卫蛋白，优化纯化工艺后获得高纯度蛋白，体外实验证明其对锌离子的强结合能力及抑制微生物生长的功能。
3. **文献名称**：Development of a recombinant calprotectin standard for improved diagnostic assays
**作者**：Kolbe, U., et al.
**摘要**：建立基于重组钙卫蛋白的标准化检测方法，解决了天然蛋白提取批次差异问题。研究显示重组蛋白在ELISA检测中表现出高灵敏度和稳定性，适用于炎症性肠病的临床诊断。
注：以上文献为示例性内容，实际文献可能需要通过PubMed或Web of Science以关键词"recombinant calprotectin"检索获取最新研究。如需具体文章，建议访问学术数据库核实。
CALPRO, commonly referring to recombinant calprotectin, is a heterodimeric protein complex composed of S100A8 and S100A9 subunits, belonging to the S100 calcium-binding protein family. Naturally expressed in neutrophils, monocytes, and epithelial cells, calprotectin plays a critical role in innate immunity by regulating inflammation, microbial growth, and cellular processes. Its name derives from its calcium-dependent antimicrobial properties and its abundance in inflammatory conditions.
Recombinant CALPRO is engineered using biotechnological platforms (e.g., E. coli or mammalian expression systems) to mimic the native protein’s structure and function. The recombinant form retains the ability to chelate essential micronutrients like zinc and manganese, thereby inhibiting bacterial and fungal growth—a key mechanism in host defense. It also interacts with Toll-like receptors (TLRs) and RAGE (receptor for advanced glycation end products), modulating immune responses.
Clinically, calprotectin serves as a biomarker for gastrointestinal inflammation, particularly in distinguishing inflammatory bowel disease (IBD) from functional disorders. Recombinant CALPRO is standardized for diagnostic assays (e.g., ELISA, fecal tests) due to its stability and reproducibility. Beyond diagnostics, it is used in research to study chronic inflammation, cancer progression (e.g., tumor microenvironment regulation), and autoimmune diseases. Its recombinant production ensures batch-to-batch consistency, enabling therapeutic exploration, such as targeting microbial resistance or modulating inflammatory pathways. Ongoing studies focus on its dual role as both a pro-inflammatory mediator and a protector in tissue repair, highlighting its therapeutic potential.
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