| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MASP1 |
| Uniprot No | P48740 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-699aa |
| 氨基酸序列 | HTVELNNMFGQIQSPGYPDSYPSDSEVTWNITVPDGFRIKLYFMHFNLESSYLCEYDYVKVETEDQVLATFCGRETTDTEQTPGQEVVLSPGSFMSITFRSDFSNEERFTGFDAHYMAVDVDECKEREDEELSCDHYCHNYIGGYYCSCRFGYILHTDNRTCRVECSDNLFTQRTGVITSPDFPNPYPKSSECLYTIELEEGFMVNLQFEDIFDIEDHPEVPCPYDYIKIKVGPKVLGPFCGEKAPEPISTQSHSVLILFHSDNSGENRGWRLSYRAAGNECPELQPPVHGKIEPSQAKYFFKDQVLVSCDTGYKVLKDNVEMDTFQIECLKDGTWSNKIPTCKIVDCRAPGELEHGLITFSTRNNLTTYKSEIKYSCQEPYYKMLNNNTGIYTCSAQGVWMNKVLGRSLPTCLPVCGLPKFSRKLMARIFNGRPAQKGTTPWIAMLSHLNGQPFCGGSLLGSSWIVTAAHCLHQSLDPEDPTLRDSDLLSPSDFKIILGKHWRLRSDENEQHLGVKHTTLHPQYDPNTFENDVALVELLESPVLNAFVMPICLPEGPQQEGAMVIVSGWGKQFLQRFPETLMEIEIPIVDHSTCQKAYAPLKKKVTRDMICAGEKEGGKDACAGDSGGPMVTLNRERGQWYLVGTVSWGDDCGKKDRYGVYSYIHHNKDWIQRVTGVRN |
| 预测分子量 | 79.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MASP1重组蛋白的3篇参考文献，按文献名称、作者和摘要内容简要概括：
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1. **"Structural and functional characterization of recombinant MASP-1 protease"**
- **作者**: Dahl MR, Thiel S, Matsushita M, et al.
- **摘要**: 该研究通过重组表达人源MASP1蛋白，解析其结构与功能，证实其在补体凝集素途径中的关键作用，包括酶活性调控及底物特异性。
2. **"MASP-1 autoactivation mechanism: Role of recombinant mutants in pathway regulation"**
- **作者**: Héja D, Kocsis A, Dobo J, et al.
- **摘要**: 利用重组MASP1突变体揭示其自身激活的分子机制，阐明补体凝集素途径的级联反应调控，并探讨其与炎症疾病的潜在关联。
3. **"Recombinant MASP-1 induces endothelial permeability and contributes to hereditary angioedema pathogenesis"**
- **作者**: Kocsis AK, Széplaki G, Dobo J, et al.
- **摘要**: 研究发现重组MASP1通过激活缓激肽通路增加血管通透性，为遗传性血管水肿(HAE)的病理机制提供了实验依据。
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以上文献聚焦于MASP1重组蛋白的结构功能、激活机制及疾病关联，涵盖基础研究与临床应用方向。如需扩展，可补充特定应用场景(如药物开发或诊断标志物)的研究。
MASP1 (Mannan-binding lectin-associated serine protease 1) is a key serine protease in the lectin pathway of the complement system, a critical component of innate immunity. Produced primarily in the liver, it circulates as a zymogen bound to pattern recognition molecules like mannose-binding lectin (MBL), ficolins, or collectins. Upon pathogen recognition, MASP1 undergoes autoactivation, initiating proteolytic cascades that cleave complement components C2 and C4 to form the C3 convertase (C4b2a), driving downstream immune responses such as opsonization, inflammation, and pathogen clearance.
Structurally, MASP1 contains six domains: a CUB1-EGF-CUB2 tandem followed by two complement control protein (CCP) domains and a C-terminal serine protease (SP) domain. Its recombinant form, generated via expression systems like mammalian or insect cells, retains enzymatic activity and structural integrity, enabling functional studies. Recombinant MASP1 is widely used to investigate lectin pathway regulation, enzyme kinetics, and interactions with inhibitors or pathogens. It also serves as a tool to explore its non-complement roles in coagulation, embryonic development, and tissue remodeling.
Dysregulation of MASP1 is linked to diseases like 3MC syndrome (a developmental disorder) and inflammatory conditions. Recombinant MASP1 proteins aid in therapeutic research, including inhibitor screening for complement-mediated disorders. Recent studies also highlight its potential as a biomarker in autoimmune and infectious diseases. By providing a controllable source of active enzyme, recombinant MASP1 accelerates mechanistic studies and drug discovery targeting the lectin pathway.
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