| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MASP2 |
| Uniprot No | O00187 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-686aa |
| 氨基酸序列 | MRLLTLLGLLCGSVATPLGPKWPEPVFGRLASPGFPGEYANDQERRWTLT APPGYRLRLYFTHFDLELSHLCEYDFVKLSSGAKVLATLCGQESTDTERA PGKDTFYSLGSSLDITFRSDYSNEKPFTGFEAFYAAEDIDECQVAPGEAP TCDHHCHNHLGGFYCSCRAGYVLHRNKRTCSALCSGQVFTQRSGELSSPE YPRPYPKLSSCTYSISLEEGFSVILDFVESFDVETHPETLCPYDFLKIQT DREEHGPFCGKTLPHRIETKSNTVTITFVTDESGDHTGWKIHYTSTAQPC PYPMAPPNGHVSPVQAKYILKDSFSIFCETGYELLQGHLPLKSFTAVCQK DGSWDRPMPACSIVDCGPPDDLPSGRVEYITGPGVTTYKAVIQYSCEETF YTMKVNDGKYVCEADGFWTSSKGEKSLPVCEPVCGLSARTTGGRIYGGQK AKPGDFPWQVLILGGTTAAGALLYDNWVLTAAHAVYEQKHDASALDIRMG TLKRLSPHYTQAWSEAVFIHEGYTHDAGFDNDIALIKLNNKVVINSNITP ICLPRKEAESFMRTDDIGTASGWGLTQRGFLARNLMYVDIPIVDHQKCTA AYEKPPYPRGSVTANMLCAGLESGGKDSCRGDSGGALVFLDSETERWFVG GIVSWGSMNCGEAGQYGVYTKVINYIPWIENIISDF |
| 预测分子量 | 102 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MASP2重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*Structural and functional characterization of recombinant MASP-2 reveals a common activation mechanism among complement enzymes*
**作者**：Thiel, S., et al.
**摘要**：该研究通过重组表达MASP-2蛋白，解析其晶体结构并分析其酶活性，揭示了MASP-2在补体凝集素途径中的活化机制，发现其与其他补体蛋白酶(如C1r/C1s)具有相似的激活过程。
2. **文献名称**：*Recombinant MASP-2 enhances lectin pathway activation and bacterial opsonization*
**作者**：Gál, P., et al.
**摘要**：研究利用重组MASP-2蛋白，验证其在体外补体凝集素途径中的功能，证明其通过切割C4和C2促进C3转化酶形成，从而增强病原体的调理吞噬作用。
3. **文献名称**：*Autoactivation of recombinant human MASP-2 facilitates structural insights into protease activity regulation*
**作者**：Héja, D., et al.
**摘要**：通过重组表达人源MASP-2.揭示了其自身活化的分子机制，并发现其活性受钙离子和配体结合调控，为靶向MASP-2的抑制剂设计提供了结构基础。
(注：上述文献信息为示例，实际引用时需核实具体来源及细节。)
MASP-2 (mannan-binding lectin-associated serine protease 2) is a key enzymatic component of the lectin pathway of the complement system, a critical arm of innate immunity. Produced primarily in the liver, MASP-2 circulates as a zymogen bound to pattern-recognition molecules like MBL (mannan-binding lectin), ficolins, or CL-K1. Its structure comprises six domains: CUB1-EGF-CUB2-CCP1-CCP2-SP, enabling calcium-dependent oligomerization and proteolytic activity. Upon pathogen-associated molecular pattern recognition, MASP-2 undergoes autoactivation, cleaving complement components C4 and C2 to form the C3 convertase (C4b2a), initiating downstream inflammatory and opsonization cascades.
Recombinant MASP-2 proteins are engineered using expression systems (e.g., mammalian, insect cells) to study its role in diseases linked to lectin pathway dysregulation. Overactivation of MASP-2 is implicated in ischemia-reperfusion injury, thrombotic microangiopathies, and autoimmune disorders, while deficiencies correlate with infection susceptibility. Therapeutic interest focuses on developing MASP-2 inhibitors; narsoplimab, a humanized monoclonal antibody, has reached clinical trials for transplant-associated thrombotic microangiopathy and IgA nephropathy.
Recombinant MASP-2 enables structural studies (e.g., crystal structures resolving activation mechanisms) and functional assays to screen modulators. Its specificity for C4/C2. distinct from classical pathway enzymes, makes it a selective therapeutic target. Challenges include preserving native conformation during production and addressing species-specific activity differences in preclinical models. Current research explores its interplay with MASP-1 (which enhances MASP-2 activation) and non-canonical roles in coagulation and embryogenesis. As a biomarker, recombinant MASP-2 aids in quantifying lectin pathway activity in inflammatory conditions, supporting personalized immunotherapeutic approaches.
×
