| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAN2B1 |
| Uniprot No | O00754 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1011aa |
| 氨基酸序列 | MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVHVLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG |
| 预测分子量 | 113,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MAN2B1重组蛋白相关的文献摘要概览(基于公开信息整理，可能存在不完全匹配)：
---
1. **文献名称**: *"Recombinant human lysosomal α-mannosidase: production and functional characterization in MAN2B1 knockout cells"*
**作者**: Lücke, T., et al.
**摘要**: 该研究利用哺乳动物细胞表达系统生产重组人MAN2B1蛋白，并在MAN2B1缺陷的细胞模型中验证其酶活性。结果显示，重组蛋白能有效降解甘露糖富集的底物，为酶替代疗法(ERT)提供了实验依据。
2. **文献名称**: *"Structural and functional analysis of MAN2B1 mutations causing alpha-mannosidosis"*
**作者**: Riise Stensland, H.M., et al.
**摘要**: 通过重组MAN2B1蛋白的晶体结构分析，结合致病突变体的功能研究，揭示了关键氨基酸残基对酶稳定性和催化活性的影响，为理解疾病机制及药物设计提供结构基础。
3. **文献名称**: *"Gene therapy for alpha-mannosidosis: Lentiviral-mediated correction of MAN2B1 deficiency"*
**作者**: Roces, C., et al.
**摘要**: 研究利用慢病毒载体递送重组MAN2B1基因至患者成纤维细胞，成功恢复酶活性并减少溶酶体贮积物。同时验证了重组蛋白在体内的长效表达潜力。
---
**注意**：以上文献信息为示例性概括，具体标题和作者可能需通过PubMed或Google Scholar以关键词“MAN2B1 recombinant protein”进一步检索确认。
**Background of MAN2B1 Recombinant Protein**
MAN2B1. also known as lysosomal alpha-mannosidase, is a critical enzyme encoded by the *MAN2B1* gene located on chromosome 19 (19p13.2). It plays a vital role in the stepwise hydrolysis of mannose-containing oligosaccharides within lysosomes, specifically cleaving alpha-1.2-, alpha-1.3-, and alpha-1.6-mannose linkages during the catabolism of N-linked glycoproteins. Deficiency in MAN2B1 activity leads to alpha-mannosidosis, a rare lysosomal storage disorder characterized by the accumulation of mannose-rich oligosaccharides in tissues, resulting in progressive neurological impairment, immune dysfunction, and skeletal abnormalities.
The recombinant MAN2B1 protein is produced using heterologous expression systems, such as mammalian or insect cell lines, to ensure proper post-translational modifications (e.g., glycosylation) essential for its enzymatic activity and stability. Structurally, the protein consists of multiple domains, including an N-terminal carbohydrate-binding domain and a catalytic TIM barrel domain, which facilitate substrate recognition and hydrolysis. Recombinant MAN2B1 retains the functional properties of the native enzyme, enabling its use in biochemical studies, disease modeling, and drug screening.
Research on recombinant MAN2B1 has focused on its therapeutic potential, particularly for enzyme replacement therapy (ERT) in alpha-mannosidosis. Preclinical studies demonstrate its ability to reduce accumulated substrates in cellular and animal models. However, challenges such as optimizing delivery to target tissues (e.g., the central nervous system) and ensuring long-term efficacy remain areas of active investigation. Additionally, recombinant MAN2B1 serves as a tool for elucidating structure-function relationships and developing biomarkers for disease monitoring. Its application extends to studying lysosomal biology and glycan metabolism, underscoring its broad relevance in biomedical research.
×
