| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPHA10 |
| Uniprot No | Q5JZY3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1008aa |
| 氨基酸序列 | METCAGPHPLRLFLCRMQLCLALLLGPWRPGTAEEVILLDSKASQAELGWTALPSNGWEEISGVDEHDRPIRTYQVCNVLEPNQDNWLQTGWISRGRGQRIFVELQFTLRDCSSIPGAAGTCKETFNVYYLETEADLGRGRPRLGGSRPRKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSRRGFHLAFQDVGACVALVSVRVYYKQCRATVRGLATFPATAAESAFSTLVEVAGTCVAHSEGEPGSPPRMHCGADGEWLVPVGRCSCSAGFQERGDFCEACPPGFYKVSPRRPLCSPCPEHSRALENASTFCVCQDSYARSPTDPPSASCTRPPSAPRDLQYSLSRSPLVLRLRWLPPADSGGRSDVTYSLLCLRCGREGPAGACEPCGPRVAFLPRQAGLRERAATLLHLRPGARYTVRVAALNGVSGPAAAAGTTYAQVTVSTGPGAPWEEDEIRRDRVEPQSVSLSWREPIPAGAPGANDTEYEIRYYEKGQSEQTYSMVKTGAPTVTVTNLKPATRYVFQIRAASPGPSWEAQSFNPSIEVQTLGEAASGSRDQSPAIVVTVVTISALLVLGSVMSVLAIWRRPCSYGKGGGDAHDEEELYFHFKVPTRRTFLDPQSCGDLLQAVHLFAKELDAKSVTLERSLGGGRFGELCCGCLQLPGRQELLVAVHMLRDSASDSQRLGFLAEALTLGQFDHSHIVRLEGVVTRGSTLMIVTEYMSHGALDGFLRRHEGQLVAGQLMGLLPGLASAMKYLSEMGYVHRGLAARHVLVSSDLVCKISGFGRGPRDRSEAVYTTMSGRSPALWAAPETLQFGHFSSASDVWSFGIIMWEVMAFGERPYWDMSGQDVIKAVEDGFRLPPPRNCPNLLHRLMLDCWQKDPGERPRFSQIHSILSKMVQDPEPPKCALTTCPRPPTPLADRAFSTFPSFGSVGAWLEALDLCRYKDSFAAAGYGSLEAVAEMTAQDLVSLGISLAEHREALLSGISALQARVLQLQGQGVQV |
| 预测分子量 | 109 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EPHA10重组蛋白的参考文献示例(内容基于公开研究整理，部分信息可能需核实)：
1. **《EphA10 promotes cell proliferation and migration through regulation of the PI3K/Akt pathway in breast cancer》**
- 作者：Li Y, et al.
- 摘要：研究通过重组EPHA10蛋白体外实验，发现其激活PI3K/Akt信号通路，促进乳腺癌细胞增殖和迁移，提示其作为潜在治疗靶点。
2. **《Recombinant expression and functional characterization of EphA10 tyrosine kinase domain》**
- 作者：Wang X, et al.
- 摘要：报道了EPHA10激酶结构域的重组表达及纯化方法，并通过体外磷酸化实验验证其激酶活性，为结构功能研究提供基础。
3. **《EphA10 antibody-drug conjugate targeting triple-negative breast cancer》**
- 作者：Smith J, et al.
- 摘要：利用重组EPHA10蛋白开发特异性抗体，偶联药物后在小鼠模型中显著抑制三阴性乳腺癌生长，展示其靶向治疗潜力。
4. **《Expression profiling of Eph receptors in prostate cancer identifies EphA10 as a novel biomarker》**
- 作者：Chen H, et al.
- 摘要：通过重组EPHA10蛋白验证其在前列腺癌中的高表达，并关联临床分期，提示其作为诊断标志物的可能性。
注：以上为模拟示例，实际文献需通过PubMed或Google Scholar等平台检索确认。建议使用关键词“EPHA10 recombinant”或“EPHA10 protein function”查找最新研究。
Eph receptor A10 (EPHA10) is a member of the Eph receptor tyrosine kinase family, which plays critical roles in cell-cell communication, tissue patterning, and developmental processes. Unlike many Eph receptors that bind membrane-anchored ephrin ligands to regulate cell adhesion, migration, and differentiation, EPHA10 exhibits unique structural and functional characteristics. It is predominantly expressed in reproductive tissues, such as the testis and placenta, under normal physiological conditions, but its dysregulation has been implicated in various cancers, including breast, prostate, and ovarian cancers. EPHA10’s overexpression in malignancies is often associated with tumor progression, metastasis, and poor prognosis, suggesting its potential as a therapeutic target or diagnostic biomarker.
Recombinant EPHA10 protein is engineered for research applications to study its biological functions and interactions. Typically produced in mammalian or insect cell systems to ensure proper post-translational modifications, the recombinant protein includes the extracellular ligand-binding domain or full-length receptor. Its production enables investigations into ligand-binding specificity, downstream signaling pathways (e.g., MAPK/ERK or PI3K/AKT), and cross-talk with other Eph receptors. Additionally, recombinant EPHA10 serves as an antigen for antibody development or a tool for high-throughput drug screening to identify inhibitors targeting its kinase activity. Recent studies also explore its role in non-cancer contexts, such as spermatogenesis and placental development. Despite its emerging significance, EPHA10’s precise mechanisms remain less understood compared to other Eph receptors, highlighting the need for further research using recombinant protein-based approaches.
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