| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRH1 |
| Uniprot No | O00482 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541aa |
| 氨基酸序列 | MSSNSDTGDL QESLKHGLTP IGAGLPDRHG SPIPARGRLV MLPKVETEAL GLARSHGEQG QMPENMQVSQ FKMVNYSYDE DLEELCPVCG DKVSGYHYGL LTCESCKGFF KRTVQNNKRY TCIENQNCQI DKTQRKRCPY CRFQKCLSVG MKLEAVRADR MRGGRNKFGP MYKRDRALKQ QKKALIRANG LKLEAMSQVI QAMPSDLTIS SAIQNIHSAS KGLPLNHAAL PPTDYDRSPF VTSPISMTMP PHGSLQGYQT YGHFPSRAIK SEYPDPYTSS PESIMGYSYM DSYQTSSPAS IPHLILELLK CEPDEPQVQA KIMAYLQQEQ ANRSKHEKLS TFGLMCKMAD QTLFSIVEWA RSSIFFRELK VDDQMKLLQN CWSELLILDH IYRQVVHGKE GSIFLVTGQQ VDYSIIASQA GATLNNLMSH AQELVAKLRS LQFDQREFVC LKFLVLFSLD VKNLENFQLV EGVQEQVNAA LLDYTMCNYP QQTEKFGQLL LRLPEIRAIS MQAEEYLYYK HLNGDVPYNN LLIEMLHAKR A |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LRH1重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*Crystal Structure of the Human LRH-1 Ligand-Binding Domain Bound to a Synthetic Agonist*
**作者**：Ortlund, E.A. et al.
**摘要**：该研究解析了人源LRH-1配体结合域(LBD)的晶体结构，利用重组蛋白在大肠杆菌中表达并纯化，揭示了合成激动剂结合后的构象变化，为LRH1的配体依赖性功能研究提供结构基础。
2. **文献名称**：*The Orphan Nuclear Receptor LRH-1 Activates the ABCG5/ABCG8 Intergenic Promoter*
**作者**：Goodwin, B. et al.
**摘要**：通过体外实验证明重组LRH1蛋白直接结合并激活胆固醇转运基因ABCG5/ABCG8的启动子，阐明了LRH1在胆固醇代谢中的转录调控机制，重组蛋白用于凝胶迁移实验(EMSA)和报告基因分析。
3. **文献名称**：*Structure and function of the nuclear receptor coactivator/DAX-1 complex*
**作者**：Sablin, E.P. et al.
**摘要**：研究利用重组LRH1与辅抑制子DAX-1共表达纯化，结合生化与结构分析，揭示了二者相互作用抑制LRH1转录活性的分子机制，为相关疾病治疗提供靶点。
4. **文献名称**：*A cell-free high-throughput assay for screening LRH-1 modulators*
**作者**：Hammes, S.R. et al.
**摘要**：开发了一种基于重组LRH1蛋白的高通量药物筛选体系，通过荧光报告系统鉴定LRH1激动剂/拮抗剂，为代谢性疾病药物研发提供平台。
这些文献涵盖了LRH1重组蛋白在结构解析、基因调控机制、相互作用研究和药物开发中的应用。
**Background of LRH1 Recombinant Protein**
Liver receptor homolog 1 (LRH-1), also known as nuclear receptor subfamily 5 group A member 2 (NR5A2), is a member of the nuclear receptor superfamily. It functions as a transcription factor regulating gene expression involved in metabolism, embryonic development, and cellular proliferation. Structurally, LRH-1 contains a conserved DNA-binding domain (DBD) that recognizes specific promoter sequences and a ligand-binding domain (LBD) that interacts with co-regulators or putative ligands. Unlike many nuclear receptors, LRH-1 was initially classified as an orphan receptor due to the lack of identified endogenous ligands, though phospholipids like phosphatidylinositol have been proposed as potential modulators.
Recombinant LRH-1 protein is engineered for in vitro studies to elucidate its molecular mechanisms, structural dynamics, and interactions. It is typically produced using expression systems such as *E. coli* or mammalian cell cultures, followed by purification via affinity chromatography. The recombinant protein retains functional domains, enabling research on its role in diseases such as cancer, metabolic disorders, and inflammation. For instance, LRH-1 overexpression is linked to tumor progression in pancreatic and breast cancers, while its dysregulation impacts bile acid homeostasis and glucose metabolism.
Studies using recombinant LRH-1 have advanced structural biology, including X-ray crystallography and cryo-EM, revealing conformational changes upon co-activator binding. Additionally, it serves as a tool for drug discovery, particularly in identifying synthetic agonists or antagonists that could modulate its activity. Challenges remain in understanding ligand specificity and tissue-specific functions, but recombinant LRH-1 remains pivotal in bridging molecular insights to therapeutic applications.
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