| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PNR |
| Uniprot No | O14804 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MRAVFIQGAEEHPAAFCYQVNGSCPRTVHTLGIQLVIYLACAAGMLIIVLGNVFVAFAVSYFKALHTPTNFLLLSLALADMFLGLLVLPLSTIRSVESCWFFGDFLCRLHTYLDTLFCLTSIFHLCFISIDRHCAICDPLLYPSKFTVRVALRYILAGWGVPAAYTSLFLYTDVVETRLSQWLEEMPCVGSCQLLLNKFWGWLNFPLFFVPCLIMISLYVKIFVVATRQAQQITTLSKSLAGAAKHERKAAKTLGIAVGIYLLCWLPFTIDTMVDSLLHFITPPLVFDIFIWFAYFNSACNPIIYVFSYQWFRKALKLTLSQKVFSPQTRTVDLYQE |
| 预测分子量 | 38,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PNR重组蛋白的3篇参考文献示例(注：文献信息为模拟示例，仅供参考)：
1. **《Expression and Purification of Recombinant Photoreceptor-Specific Nuclear Receptor (PNR) in Escherichia coli》**
- **作者**: Smith J. et al. (2015)
- **摘要**: 本研究报道了在大肠杆菌系统中高效表达并纯化PNR重组蛋白的方法，通过优化诱导条件和亲和层析技术获得高纯度蛋白，并验证了其体外结合特定DNA序列的能力。
2. **《Structural Insights into PNR Ligand Binding Domain Using X-ray Crystallography》**
- **作者**: Lee S. et al. (2020)
- **摘要**: 利用重组PNR蛋白的配体结合域(LBD)，通过哺乳动物细胞表达系统获得可溶性蛋白，解析了其晶体结构，揭示了与视黄酸类配体的相互作用机制。
3. **《Functional Analysis of PNR in Retinal Development via Recombinant Protein Interaction Screening》**
- **作者**: Zhang Y. et al. (2018)
- **摘要**: 采用杆状病毒-昆虫细胞系统表达PNR重组蛋白，结合蛋白质互作组学技术，鉴定出多个与视网膜发育相关的相互作用蛋白，阐明PNR在光感受器分化中的调控网络。
提示：实际文献检索建议使用PubMed或Google Scholar，以关键词“Photoreceptor-specific nuclear receptor recombinant”或“PNR protein purification”进行查询，并筛选实验类研究论文。
**Background of PNR Recombinant Protein**
The photoreceptor-specific nuclear receptor (PNR), also known as NR2E3. is a transcription factor belonging to the nuclear receptor superfamily. It plays a critical role in the development and maintenance of retinal photoreceptor cells, particularly rod and cone cells in the retina. PNR regulates the expression of genes essential for photoreceptor function, including those involved in phototransduction, cell differentiation, and retinal homeostasis. Structurally, it contains a DNA-binding domain (DBD) and a ligand-binding domain (LBD), though its endogenous ligand remains unidentified.
Mutations in the *NR2E3* gene are linked to inherited retinal diseases, such as enhanced S-cone syndrome (ESCS) and retinitis pigmentosa (RP), highlighting its importance in retinal health. To study PNR’s molecular mechanisms and disease-related pathways, recombinant PNR proteins are engineered using expression systems like *E. coli* or mammalian cells. These recombinant proteins retain functional domains, enabling in vitro studies on DNA binding, protein interactions, and transcriptional regulation.
The production of PNR recombinant protein facilitates drug screening, structural analyses (e.g., X-ray crystallography), and the development of gene therapies targeting retinal degeneration. Challenges include ensuring proper folding and post-translational modifications, which often necessitate mammalian expression systems. Current research focuses on leveraging recombinant PNR to decipher its role in photoreceptor development and to design therapeutic strategies for vision disorders. Overall, PNR recombinant proteins serve as vital tools for advancing retinal biology and translational ophthalmology.
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