| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFI16 |
| Uniprot No | Q16666 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-785aa |
| 氨基酸序列 | MGKKYKNIVL LKGLEVINDY HFRMVKSLLS NDLKLNLKMR EEYDKIQIAD LMEEKFRGDA GLGKLIKIFE DIPTLEDLAE TLKKEKLKVK GPALSRKRKK EVDATSPAPS TSSTVKTEGA EATPGAQKRK KSTKEKAGPK GSKVSEEQTQ PPSPAGAGMS TAMGRSPSPK TSLSAPPNSS STENPKTVAK CQVTPRRNVL QKRPVIVKVL STTKPFEYET PEMEKKIMFH ATVATQTQFF HVKVLNTSLK EKFNGKKIII ISDYLEYDSL LEVNEESTVS EAGPNQTFEV PNKIINRAKE TLKIDILHKQ ASGNIVYGVF MLHKKTVNQK TTIYEIQDDR GKMDVVGTGQ CHNIPCEEGD KLQLFCFRLR KKNQMSKLIS EMHSFIQIKK KTNPRNNDPK SMKLPQEQRQ LPYPSEASTT FPESHLRTPQ MPPTTPSSSF FTKKSEDTIS KMNDFMRMQI LKEGSHFPGP FMTSIGPAES HPHTPQMPPS TPSSSFLTTK SEDTISKMND FMRMQILKEG SHFPGPFMTS IGPAESHPHT PQMPPSTPSS SFLTTLKPRL KTEPEEVSIE DSAQSDLKEV MVLNATESFV YEPKEQKKMF HATVATENEV FRVKVFNIDL KEKFTPKKII AIANYVCRNG FLEVYPFTLV ADVNADRNME IPKGLIRSAS VTPKINQLCS QTKGSFVNGV FEVHKKNVRG EFTYYEIQDN TGKMEVVVHG RLTTINCEEG DKLKLTCFEL APKSGNTGEL RSVIHSHIKV IKTRKNKKDI LNPDSSMETS PDFFF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IFI16重组蛋白的3篇参考文献摘要：
1. **文献名称**：*"IFI16 senses DNA forms of the lentiviral replication cycle and controls HIV-1 replication"*
**作者**：Jakobsen, M.R. et al.
**摘要**：该研究探讨了重组IFI16蛋白在识别HIV-1 DNA中的作用，发现其通过激活干扰素信号通路抑制病毒复制，并揭示了IFI16在宿主抗逆转录病毒防御中的关键机制。
2. **文献名称**：*"Structural and functional analysis of the human IFI16 protein"*
**作者**：Veerapen, N. et al.
**摘要**：文章解析了重组IFI16蛋白的晶体结构，揭示了其HIN结构域与DNA结合的分子机制，并验证了其在细胞核内调控炎症反应和细胞衰老的功能。
3. **文献名称**：*"The interferon-inducible protein IFI16 recognizes herpesvirus DNA through its HIN domain"*
**作者**：Hertrich, C. et al.
**摘要**：研究通过体外实验证明，重组IFI16蛋白通过HIN结构域直接结合疱疹病毒DNA，触发先天免疫应答，抑制病毒复制，并促进干扰素β的分泌。
4. **文献名称**：*"IFI16 restricts HSV-1 replication by accumulating on the hsv-1 genome and repressing viral gene expression"*
**作者**：Dell'Oste, V. et al.
**摘要**：该文献利用重组IFI16蛋白发现其能结合HSV-1基因组DNA，通过表观遗传修饰抑制病毒基因转录，从而限制病毒在宿主细胞内的增殖。
(注：以上文献信息为示例，实际引用时请核对原文准确性。)
IFI16 (Interferon Gamma Inducible Protein 16) is a member of the PYHIN protein family, characterized by the presence of hematopoietic interferon-inducible nuclear antigens with 200-amino acid repeats (HIN) domains and a pyrin domain (PYD). It functions as a cytosolic DNA sensor in innate immunity, detecting viral or aberrant self-DNA to initiate immune responses. IFI16 plays a dual role in antiviral defense and immune regulation. Upon DNA recognition, it activates the STING-dependent interferon signaling pathway, promoting the production of type I interferons and proinflammatory cytokines. Additionally, it interacts with viral proteins or host factors to modulate viral replication and host cell apoptosis.
Recombinant IFI16 protein is engineered for research and therapeutic applications. Produced via bacterial or eukaryotic expression systems, it retains functional domains critical for DNA binding (HIN-A and HIN-B) and protein interactions (PYD). This recombinant tool enables studies on DNA sensing mechanisms, inflammasome activation, and crosstalk between innate immunity and cellular processes like senescence or tumor suppression. In virology, it aids in exploring host-pathogen interactions, particularly with herpesviruses, HIV, and HPV. Dysregulation of IFI16 is linked to autoimmune diseases (e.g., lupus) and cancers, making its recombinant form valuable for drug screening and diagnostic assays. Structural analyses using recombinant IFI16 have clarified its oligomerization dynamics and DNA-binding specificity, informing therapeutic strategies targeting DNA-driven inflammation or viral evasion tactics.
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