| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GADL1 |
| Uniprot No | Q6ZQY3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-521aa |
| 氨基酸序列 | MSSDSDRQCP VDGDIDQQEM IPSKKNAVLV DGVVLNGPTT DAKAGEKFVE EACRLIMEEV VLKATDVNEK VCEWRPPEQL KQLLDLEMRD SGEPPHKLLE LCRDVIHYSV KTNHPRFFNQ LYAGLDYYSL VARFMTEALN PSVYTYEVSP VFLLVEEAVL KKMIEFIGWK EGDGIFNPGG SVSNMYAMNL ARYKYCPDIK EKGLSGSPRL ILFTSAECHY SMKKAASFLG IGTENVCFVE TDGRGKMIPE ELEKQVWQAR KEGAAPFLVC ATSGTTVLGA FDPLDEIADI CERHSLWLHV DASWGGSALM SRKHRKLLHG IHRADSVAWN PHKMLMAGIQ CCALLVKDKS DLLKKCYSAK ASYLFQQDKF YDVSYDTGDK SIQCSRRPDA FKFWMTWKAL GTLGLEERVN RALALSRYLV DEIKKREGFK LLMEPEYANI CFWYIPPSLR EMEEGPEFWA KLNLVAPAIK ERMMKKGSLM LGYQPHRGKV NFFRQVVISP QVSREDMDFL LDEIDLLGKD M |
| 预测分子量 | 59,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GADL1重组蛋白的3篇代表性文献(虚拟示例，供参考)：
1. **"Functional characterization of recombinant human GADL1 enzyme"**
- **作者**: Lee S, et al.
- **摘要**: 通过大肠杆菌表达系统成功纯化GADL1重组蛋白，证实其具有催化半胱氨酸亚磺酸生成牛磺酸的酶活性，并揭示其与GAD65/67不同的底物特异性。
2. **"Structural insights into GADL1 through recombinant protein crystallization"**
- **作者**: Zhang Y, et al.
- **摘要**: 利用重组GADL1蛋白解析其三维晶体结构，发现其活性中心独特的氨基酸残基排列，为理解其催化机制及药物靶点设计提供依据。
3. **"GADL1 recombinant protein modulates calcium signaling in cardiomyocytes"**
- **作者**: Wang H, et al.
- **摘要**: 体外实验表明，重组GADL1蛋白通过调控牛磺酸合成影响心肌细胞钙离子稳态，提示其在心血管疾病中的潜在作用。
注：以上为模拟文献，实际研究中请通过PubMed或SciHub等平台检索具体论文。
**Background of GADL1 Recombinant Protein**
Glutamate decarboxylase-like 1 (GADL1) is a member of the glutamate decarboxylase (GAD) enzyme family, which shares structural homology with the well-characterized isoforms GAD65 and GAD67. However, unlike these isoforms—known for catalyzing the conversion of glutamate to gamma-aminobutyric acid (GABA), a key inhibitory neurotransmitter—GADL1 exhibits distinct biochemical properties and tissue-specific expression. Initially identified through genomic studies, GADL1 is highly expressed in the kidneys, testes, and skeletal muscle, suggesting roles beyond neurotransmission.
While GADL1 retains the conserved pyridoxal phosphate (PLP)-binding domain characteristic of GAD enzymes, its catalytic activity remains debated. Some studies propose it may metabolize alternative substrates or function as a regulatory protein in cellular processes such as energy metabolism or ion transport. Notably, GADL1 has been linked to human diseases, including cardiovascular conditions and metabolic disorders, though its precise mechanisms remain under investigation.
Recombinant GADL1 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Purification often involves affinity tags (e.g., His-tag) and chromatography techniques. This engineered protein serves as a critical tool for exploring GADL1’s enzymatic activity, substrate specificity, and interactions with potential inhibitors or binding partners. Additionally, it aids in antibody development for diagnostic applications and high-throughput drug screening campaigns targeting GADL1-associated pathways.
Research on GADL1 recombinant protein continues to advance our understanding of its physiological roles and therapeutic potential, bridging gaps between molecular biology and clinical applications.
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