| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARNTL |
| Uniprot No | O00327 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-625aa |
| 氨基酸序列 | MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESM DTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVP TCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHL ILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKD IAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCR MKCNRPSVKVEDKDFPSTCSKKKDRKSFCTIHSTGYLKSWPPTKMGLDED NEPDNEGCNLSCLVAIGRLHSHVVPQPVNGEIRVKSMEYVSRHAIDGKFV FVDQRATAILAYLPQELLGTSCYEYFHQDDIGHLAECHRQVLQTREKITT NCYKFKIKDGSFITLRSRWFSFMNPWTKEVEYIVSTNTVVLANVLEGGDP TFPQLTASPHSMDSMLPSGEGGPKRTHPTVPGIPGGTRAGAGKIGRMIAE EIMEIHRIRGSSPSSCGSSPLNITSTPPPDASSPGGKKILNGGTPDIPSS GLLSGQAQENPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMA VIMSLLEADAGLGGPVDFSDLPWPL |
| 预测分子量 | 94 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARNTL(BMAL1)重组蛋白的3篇参考文献的简要概括：
1. **文献名称**：*Crystal Structure of the Heterodimeric CLOCK:BMAL1 Transcriptional Activator Complex*
**作者**：Huang, N., et al.
**摘要**：该研究解析了CLOCK:BMAL1异源二聚体的晶体结构，揭示了二者通过PAS结构域相互作用，并阐明了其在昼夜节律调控中招募辅因子的分子机制。
2. **文献名称**：*Functional Analysis of Recombinant BMAL1 Protein in Circadian Clock Regulation*
**作者**：Takahashi, J.S., et al.
**摘要**：通过体外重组表达BMAL1蛋白，研究证实其与CLOCK蛋白结合后激活下游靶基因(如Per和Cry)的转录，并验证了其DNA结合域的关键作用。
3. **文献名称**：*Post-translational Modifications of BMAL1 Regulate Circadian Rhythms*
**作者**：Gallego, M., & Virshup, D.M.
**摘要**：该文献探讨了重组BMAL1蛋白的磷酸化、泛素化修饰对其稳定性及昼夜节律周期的影响，揭示了翻译后修饰在生物钟精准性中的调控机制。
(注：以上文献标题及作者为示例性质，具体文献需根据实际研究检索确认。)
**Background of ARNTL Recombinant Protein**
ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like), also known as BMAL1 (Brain and Muscle ARNT-Like 1), is a core circadian rhythm regulator encoded by the *ARNTL* gene. It functions as a transcription factor within the circadian clock machinery, forming a heterodimer with CLOCK (Circadian Locomotor Output Cycles Kaput) to drive the expression of circadian-controlled genes, including *Period (PER)* and *Cryptochrome (CRY)*, which feedback to inhibit ARNTL/CLOCK activity, establishing a 24-hour oscillatory loop.
Recombinant ARNTL protein is engineered for *in vitro* studies to dissect molecular mechanisms of circadian regulation. It is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity tags (e.g., Poly-His). The recombinant protein retains functional domains, including basic helix-loop-helix (bHLH) and PAS domains critical for dimerization and DNA binding.
Research applications include investigating circadian disruptions in metabolic disorders, cancer, and neurological diseases. For example, ARNTL dysfunction is linked to sleep disorders, insulin resistance, and tumor progression. Recombinant ARNTL enables *in vitro* assays (e.g., electrophoretic mobility shift assays) to study DNA-binding kinetics or screen small molecules targeting circadian pathways for therapeutic development.
Quality control involves verifying molecular weight (~60-70 kDa), purity (SDS-PAGE), and functional activity (e.g., dimerization with CLOCK). Challenges include maintaining protein stability due to its large size and post-translational modification sensitivity. Nonetheless, recombinant ARNTL remains a vital tool for unraveling circadian biology and its implications in human health.
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