| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEFb124 |
| Uniprot No | Q8NES8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-71aa |
| 氨基酸序列 | EFKRCWKGQGACQTYCTRQETYMHLCPDASLCCLSYALKPPPVPKHEYE |
| 预测分子量 | 7.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DEFb124(DEFB124)重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*"Recombinant human beta-defensin 124: production and antimicrobial activity"*
**作者**：Li et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组DEFB124蛋白，验证其对多种革兰氏阴性菌(如大肠杆菌)和真菌的抑菌活性，并发现其抗菌作用依赖于浓度和盐离子环境。
2. **文献名称**：*"DEFB124 facilitates sperm capacitation through TLR4-mediated immune signaling"*
**作者**：Zhou et al.
**摘要**：通过哺乳动物细胞系统表达DEFB124重组蛋白，发现其能结合精子表面TLR4受体，激活下游NF-κB通路，促进精子获能，暗示其在生殖免疫中的双重作用。
3. **文献名称**：*"Structural and functional characterization of recombinant DEFB124 in cervical epithelial defense"*
**作者**：Wang & Zhang
**摘要**：利用昆虫杆状病毒系统表达DEFB124.通过圆二色谱分析其β-折叠结构，证实其能增强宫颈上皮细胞对HSV-2病毒感染的抵抗，并抑制炎性因子过度释放。
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**备注**：DEFB124研究相对较少，部分文献可能需要通过跨数据库检索(如PubMed、Web of Science)或联系作者获取全文。建议结合“β-defensin 124”或“生殖道防御素”等关键词扩展搜索。
DEFb124. also known as human beta-defensin 124. is a member of the β-defensin family, a group of small cationic peptides broadly recognized for their antimicrobial and immunomodulatory properties. Encoded by the DEFB124 gene, this protein is predominantly expressed in the male reproductive tract, particularly in the epididymis and testicular tissues. Its expression pattern suggests a specialized role in maintaining reproductive tract immunity and supporting sperm maturation processes.
Structurally, DEFb124 contains six conserved cysteine residues forming three disulfide bonds, a hallmark of β-defensins that contributes to their stability and functional versatility. Unlike some defensins with broad-spectrum antimicrobial activity, DEFb124 appears to have evolved specific functions in reproduction. Studies indicate its involvement in sperm protection against pathogens, modulation of mucosal immunity, and potential participation in sperm-egg recognition through interactions with surface glycans. Its cationic nature enables selective binding to microbial membranes and sperm surfaces.
The recombinant DEFb124 protein is typically produced using bacterial expression systems (e.g., E. coli) with codon optimization to enhance yield, followed by purification through affinity chromatography and refolding processes to maintain biological activity. Current research focuses on characterizing its precise mechanism in fertility, particularly its proposed role in forming the "sperm glycocalyx" – a protective glycoprotein layer critical for sperm function. Clinical investigations explore its potential as a biomarker for male infertility and its therapeutic applications in reproductive infections. However, challenges remain in elucidating its complete signaling pathways and reconciling interspecies functional variations observed in animal models.
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