| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPIN3 |
| Uniprot No | Q5JUX0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-258aa |
| 氨基酸序列 | MKTPFGKAAA GQRSRTGAGH GSVSVTMIKR KAAHKKHRSR PTSQPRGNIV GCRIQHGWKD GDEPLTQWKG TVLDQVPVNP SLYLIKYDGF DCVYGLELHR DERVSSLEVL PNRVASSRIS DTHLAEIMVG KAVEHIFETE EGSKNEWRGM VLAQAPVMNT WFYITYEKDP VLYMYQLLDD YKDGDLRILQ DSNDSPLAER EPGEVIDSLV GKQVEYAKDD GSKRTGMVIH QVEAKPSVYF IKFDDDFHIY VYDLVKTS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPIN3重组蛋白的3篇参考文献示例(注：部分文献为模拟示例，实际引用时请核实真实性和数据库检索结果)：
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1. **文献名称**：*Expression and Purification of Recombinant SPIN3 in Escherichia coli for Structural Studies*
**作者**：Zhang Y, Wang L, Liu X
**摘要**：该研究报道了利用大肠杆菌系统高效表达SPIN3重组蛋白的优化策略，通过His标签亲和层析纯化获得高纯度蛋白，并验证其可用于后续X射线晶体学分析。
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2. **文献名称**：*SPIN3 Recombinant Protein Binds to Histone H3K4me3 and Modulates Epigenetic Regulation in Cancer Cells*
**作者**：Kim S, Park JH, Lee M
**摘要**：研究团队成功表达并纯化了人源SPIN3重组蛋白，证实其通过特异性识别组蛋白H3K4三甲基化修饰(H3K4me3)，在癌细胞中参与表观遗传调控及肿瘤发生机制。
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3. **文献名称**：*Functional Characterization of Recombinant SPIN3 in Neuronal Autophagy Pathways*
**作者**：Chen R, Xu T, Zhou D
**摘要**：通过哺乳动物细胞系表达SPIN3重组蛋白，研究发现其通过结合自噬关键蛋白LC3.调控神经元自噬活性，为神经退行性疾病治疗提供潜在靶点。
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**注**：以上文献为示例性质，若需真实文献，建议在PubMed、Web of Science等平台检索关键词“SPIN3 recombinant protein”或结合具体研究领域筛选。
**Background of SPIN3 Recombinant Protein**
SPIN3. also known as Spindlin3 or SSTY3. belongs to the SPIN/SSTY protein family, which is characterized by tandem Tudor domains and a conserved SPIN/SSTY structural motif. This family is implicated in diverse cellular processes, including chromatin remodeling, transcriptional regulation, and germ cell development. SPIN3. specifically, is recognized for its role in binding methylated histone marks, particularly trimethylated lysine 4 on histone H3 (H3K4me3), a hallmark of transcriptionally active chromatin. This interaction suggests its involvement in epigenetic regulation, potentially influencing gene expression during early embryogenesis and stem cell differentiation.
Structurally, SPIN3 contains three Tudor domains that facilitate its binding to methylated histones and other epigenetic modifiers. Studies highlight its expression in germ cells, embryonic tissues, and certain cancers, where it may contribute to oncogenesis by dysregulating cell cycle progression or promoting pluripotency. For instance, SPIN3 overexpression has been linked to tumor aggressiveness in hepatocellular carcinoma and glioblastoma, underscoring its potential as a therapeutic target.
Recombinant SPIN3 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production for functional studies. Its applications span *in vitro* assays to investigate histone-binding mechanisms, enzymatic activity (e.g., interactions with chromatin-modifying enzymes), and cellular models to explore its role in development and disease. Additionally, recombinant SPIN3 serves as an antigen for antibody development and a tool for high-throughput drug screening to identify inhibitors targeting its oncogenic functions.
Ongoing research aims to elucidate SPIN3's precise molecular mechanisms and its crosstalk with signaling pathways, such as Wnt/β-catenin, which may further clarify its impact on stemness and malignancy. These insights could advance regenerative medicine and cancer therapy strategies.
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