| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMD6 |
| Uniprot No | Q15008 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MPLENLEEEG LPKNPDLRIA QLRFLLSLPE HRGDAAVRDE LMAAVRDNNM APYYEALCKS LDWQIDVDLL NKMKKANEDE LKRLDEELED AEKNLGESEI RDAMMAKAEY LCRIGDKEGA LTAFRKTYDK TVALGHRLDI VFYLLRIGLF YMDNDLITRN TEKAKSLIEE GGDWDRRNRL KVYQGLYCVA IRDFKQAAEL FLDTVSTFTS YELMDYKTFV TYTVYVSMIA LERPDLREKV IKGAEILEVL HSLPAVRQYL FSLYECRYSV FFQSLAVVEQ EMKKDWLFAP HYRYYVREMR IHAYSQLLES YRSLTLGYMA EAFGVGVEFI DQELSRFIAA GRLHCKIDKV NEIVETNRPD SKNWQYQETI KKGDLLLNRV QKLSRVINM |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMD6重组蛋白的参考文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Structural and Functional Characterization of Recombinant PSMD6 in the 26S Proteasome Assembly*
**作者**：Chen, L.; Wang, X.; Zhang, Y.
**摘要**：本研究通过大肠杆菌表达系统成功纯化重组人源PSMD6蛋白，并利用X射线晶体学解析其三维结构。研究揭示了PSMD6在19S调节颗粒中的关键作用，并通过体外实验证明其参与蛋白酶体组装及底物识别。
2. **文献名称**：*PSMD6 Recombinant Protein Enhances Ubiquitin-Dependent Protein Degradation in Cancer Cells*
**作者**：Kim, H.; Park, S.; Lee, J.
**摘要**：作者通过哺乳动物细胞表达系统制备了高纯度PSMD6重组蛋白，并发现其在体外泛素化降解通路中显著促进肿瘤抑制蛋白p53的降解。研究为PSMD6在癌症治疗中的潜在靶点提供了实验依据。
3. **文献名称**：*Development of a PSMD6 Knockdown Model Using Recombinant Protein-Based Antibody Screening*
**作者**：Müller, R.; Schmidt, F.
**摘要**：该研究利用重组PSMD6蛋白筛选特异性抗体，成功构建了PSMD6功能抑制的细胞模型。实验表明，PSMD6缺失导致蛋白酶体活性下降，并影响细胞周期调控。
4. **文献名称**：*Interactome Analysis of PSMD6 Reveals Novel Binding Partners via Yeast Two-Hybrid Assay*
**作者**：Gupta, S.; Rao, A.
**摘要**：通过表达重组PSMD6蛋白进行酵母双杂交实验，鉴定出多个与PSMD6相互作用的泛素连接酶。研究拓展了对蛋白酶体调控网络的理解，强调了PSMD6在底物特异性中的调控功能。
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建议通过PubMed、Google Scholar等平台，以关键词“PSMD6 recombinant protein”、“PSMD6 proteasome”或“PSMD6 expression”检索最新文献以获取真实数据。
PSMD6 (Proteasome 26S Subunit, Non-ATPase 6) is a critical component of the 26S proteasome, a large multiprotein complex responsible for targeted protein degradation in eukaryotic cells. As part of the 19S regulatory particle, PSMD6 resides within the "lid" subcomplex, which facilitates substrate recognition, deubiquitination, and translocation of ubiquitinated proteins into the proteolytic core. This ATP-dependent process is essential for maintaining cellular homeostasis, regulating cell cycle progression, and degrading misfolded proteins.
Recombinant PSMD6 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian systems with affinity tags (e.g., His-tag) for purification. Its production enables functional studies on proteasome assembly, substrate specificity, and regulatory mechanisms. Researchers utilize PSMD6 recombinant proteins to investigate interactions with other proteasomal subunits (e.g., PSMC5/Rpt6) or ubiquitin receptors, shedding light on diseases linked to proteasome dysfunction, such as neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s) and cancers. Overexpression of PSMD6 has been observed in certain malignancies, suggesting its potential as a therapeutic target. Additionally, recombinant PSMD6 serves as an antigen for antibody development or a tool for high-throughput drug screening aimed at modulating proteasome activity. Studies also explore its role in immune responses, as proteasomes generate antigenic peptides presented by MHC class I molecules. The structural and functional insights derived from PSMD6 recombinant proteins continue to advance precision medicine strategies targeting the ubiquitin-proteasome system.
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