| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NME6 |
| Uniprot No | O75414 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-186aa |
| 氨基酸序列 | MASILRSPQA LQLTLALIKP DAVAHPLILE AVHQQILSNK FLIVRMRELL WRKEDCQRFY REHEGRFFYQ RLVEFMASGP IRAYILAHKD AIQLWRTLMG PTRVFRARHV APDSIRGSFG LTDTRNTTHG SDSVVSASRE IAAFFPDFSE QRWYEEEEPQ LRCGPVCYSP EGGVHYVAGT GGLGPA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NME6重组蛋白的3篇参考文献示例：
1. **《Functional characterization of recombinant NME6: a role in mitochondrial dynamics》**
- 作者：Zhang L, et al.
- 摘要：本研究成功在大肠杆菌中表达并纯化了重组NME6蛋白，证实其具有核苷二磷酸激酶(NDPK)活性，并发现NME6通过调控线粒体膜蛋白DRP1的磷酸化参与线粒体分裂过程。
2. **《Structural insights into NME6 oligomerization and nucleotide binding specificity》**
- 作者：Chen R, et al.
- 摘要：通过X射线晶体学解析了重组NME6蛋白的三维结构，揭示了其独特的六聚体组装模式，并阐明了其与ATP/GTP结合的特异性位点，为理解NME家族蛋白的功能多样性提供了结构基础。
3. **《NME6 interacts with tau protein and modulates its aggregation in neurodegenerative models》**
- 作者：Kim S, et al.
- 摘要：研究发现重组NME6蛋白与tau蛋白直接相互作用，通过增强tau的磷酸酶活性抑制其异常聚集，提示NME6可能在阿尔茨海默病等tau蛋白病变中具有保护作用。
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注：以上文献为模拟示例，实际研究需参考具体数据库(如PubMed)的权威论文。
NME6 (non-metastatic protein 6), also known as NM23-H6. is a member of the NME/NM23 nucleoside diphosphate kinase (NDPK) family, which plays roles in nucleotide metabolism, cellular signaling, and cytoskeletal dynamics. This family comprises ten isoforms in humans, with NME6 distinguished by its unique subcellular localization and functional specialization. Unlike the well-studied NME1 and NME2. which are linked to metastasis suppression and NDPK activity, NME6 is primarily associated with mitochondria and cilia-related processes. It is implicated in regulating mitochondrial membrane dynamics, energy homeostasis, and cilia formation, suggesting a role in cellular energy distribution and structural organization.
Recombinant NME6 protein is engineered for in vitro studies to dissect its biochemical properties and interactions. Produced using expression systems like *E. coli* or mammalian cells, the recombinant form retains key functional domains, including the conserved NDPK catalytic site and a mitochondrial targeting sequence. Researchers utilize this protein to explore its enzymatic activity, structural stability, and binding partners, particularly in mitochondrial fusion-fission dynamics and ciliary function. Dysregulation of NME6 has been tentatively linked to ciliopathies and metabolic disorders, though its mechanisms remain less defined compared to other isoforms. Current studies focus on clarifying its role in diseases involving mitochondrial dysfunction or impaired cilia signaling. By leveraging recombinant NME6. scientists aim to uncover therapeutic targets for conditions like neurodegenerative diseases or genetic syndromes tied to cellular energy dysregulation. Its study bridges gaps in understanding how NDPK family diversity translates to specialized biological functions.
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