| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SORT1 |
| Uniprot No | Q99523 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 203-299aa |
| 氨基酸序列 | FAKNFVQTDLPFHPLTQMMYSPQNSDYLLALSTENGLWVSKNFGGKWEEI HKAVCLAKWGSDNTIFFTTYANGSCKADLGALELWRTSDLGKSFKTI |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SORT1重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Sortilin/Neurotensin Receptor-3: A Key Regulator of Glial Cell Plasticity*
**作者**：Andersen, O.M. 等
**摘要**：该研究通过重组SORT1蛋白揭示了其与神经生长因子(proNGF)的相互作用机制，证实SORT1在神经元凋亡和神经退行性疾病中通过调控神经营养因子信号通路发挥关键作用。
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2. **文献名称**：*Sortilin Mediates Apoptotic Pathways via Interaction with Progranulin and TNF Receptors*
**作者**：Nykjaer, A. 等
**摘要**：利用重组SORT1蛋白进行体外结合实验，发现其与肿瘤坏死因子受体(TNFR)和颗粒蛋白前体(progranulin)的结合能力，阐明SORT1在细胞凋亡和炎症反应中的双重调控功能。
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3. **文献名称**：*Structural Basis for Ligand Recognition by the SORT1 Receptor*
**作者**：Quistgaard, E.M. 等
**摘要**：通过X射线晶体学解析重组SORT1蛋白的三维结构，揭示了其与脂蛋白颗粒(如APOE)及神经肽结合的分子机制，为靶向SORT1的代谢性疾病治疗提供了结构基础。
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以上文献涵盖了SORT1重组蛋白的功能机制、疾病关联及结构解析方向的研究，可为进一步探索其生物学作用提供参考。
**Background of SORT1 Recombinant Protein**
Sortilin 1 (SORT1), encoded by the *SORT1* gene, is a transmembrane receptor belonging to the vacuolar protein sorting 10 protein (VPS10P) domain receptor family. It plays a critical role in intracellular protein trafficking, lipid metabolism, and cellular signaling. Structurally, SORT1 contains an N-terminal VPS10P domain responsible for ligand binding, a single transmembrane domain, and a short cytoplasmic tail mediating endocytic sorting.
SORT1 is predominantly localized in the Golgi apparatus, endosomes, and plasma membrane, where it acts as a sorting receptor to shuttle proteins between these compartments. It interacts with ligands such as neurotrophins (e.g., pro-nerve growth factor), lipoproteins, and receptors like the low-density lipoprotein receptor (LDLR). Notably, SORT1 regulates LDL cholesterol metabolism by influencing hepatic LDLR degradation, linking it to cardiovascular disease risk. Genetic studies highlight *SORT1* locus variants (e.g., rs646776) as significant markers for coronary artery disease and myocardial infarction.
In neurodegenerative contexts, SORT1 mediates the trafficking of pro-apoptotic ligands (e.g., pro-BDNF, pro-NGF) and amyloid precursor protein (APP), implicating it in Alzheimer’s and Parkinson’s diseases. It also exhibits dual roles in cancer, either promoting apoptosis or supporting tumor survival, depending on cellular context.
**Recombinant SORT1 protein**, produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells), retains these functional properties and is widely used to study SORT1-ligand interactions, signaling pathways, and therapeutic targeting. Its applications include *in vitro* binding assays, drug screening for metabolic or neurodegenerative disorders, and development of SORT1-targeted therapies (e.g., antibodies, small molecules). Research continues to explore its potential as a biomarker or therapeutic target in cardiovascular, neurological, and oncological diseases.
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