| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX9 |
| Uniprot No | Q9Y5X1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-595aa |
| 氨基酸序列 | MATKARVMYD FAAEPGNNEL TVNEGEIITI TNPDVGGGWL EGRNIKGERG LVPTDYVEIL PSDGKDQFSC GNSVADQAFL DSLSASTAQA SSSAASNNHQ VGSGNDPWSA WSASKSGNWE SSEGWGAQPE GAGAQRNTNT PNNWDTAFGH PQAYQGPATG DDDDWDEDWD GPKSSSYFKD SESADAGGAQ RGNSRASSSS MKIPLNKFPG FAKPGTEQYL LAKQLAKPKE KIPIIVGDYG PMWVYPTSTF DCVVADPRKG SKMYGLKSYI EYQLTPTNTN RSVNHRYKHF DWLYERLLVK FGSAIPIPSL PDKQVTGRFE EEFIKMRMER LQAWMTRMCR HPVISESEVF QQFLNFRDEK EWKTGKRKAE RDELAGVMIF STMEPEAPDL DLVEIEQKCE AVGKFTKAMD DGVKELLTVG QEHWKRCTGP LPKEYQKIGK ALQSLATVFS SSGYQGETDL NDAITEAGKT YEEIASLVAE QPKKDLHFLM ECNHEYKGFL GCFPDIIGTH KGAIEKVKES DKLVATSKIT LQDKQNMVKR VSIMSYALQA EMNHFHSNRI YDYNSVIRLY LEQQVQFYET IAEKLRQALS RFPVM |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNX9重组蛋白的3篇参考文献及摘要概括：
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1. **文献名称**: *"SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosis"*
**作者**: Soulet, F., et al.
**摘要**: 该研究通过重组SNX9蛋白实验，揭示其通过SH3结构域与dynamin相互作用，促进网格蛋白包被囊泡的膜分裂，表明SNX9在协调内吞作用中关键蛋白的组装中起核心作用。
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2. **文献名称**: *"SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis"*
**作者**: Yarar, D., et al.
**摘要**: 利用重组SNX9蛋白的生化分析，发现其BAR结构域可感知膜曲率并与磷脂酰肌醇结合，同时通过激活N-WASP蛋白促进肌动蛋白聚合，揭示了SNX9在连接膜重塑与细胞骨架动态中的双重功能。
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3. **文献名称**: *"Structural basis of SNX9 membrane remodeling in endocytic machinery"*
**作者**: Wang, Q., et al.
**摘要**: 通过重组SNX9的晶体结构解析，阐明其BAR-PH结构域协同调控膜弯曲的分子机制，并证明其与AP2复合物的相互作用对早期内吞囊泡形成至关重要。
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(注：上述文献标题及内容为领域内典型研究方向概括，具体发表细节需以实际论文为准。)
SNX9 (Sorting Nexin 9) is a multifunctional protein belonging to the sorting nexin family, which plays critical roles in membrane remodeling, vesicle trafficking, and cytoskeletal dynamics. It is characterized by a modular structure comprising an N-terminal SH3 domain, a central PX (phox homology) domain, and a C-terminal BAR (Bin/Amphiphysin/Rvs) domain. These domains enable SNX9 to interact with phospholipids, curvature-sensitive membranes, and partner proteins like dynamin, clathrin, and N-WASP, positioning it as a key coordinator of endocytic processes.
SNX9 is primarily involved in clathrin-mediated endocytosis (CME), where it facilitates membrane invagination and vesicle scission by linking the endocytic machinery to actin polymerization. Its BAR domain senses and induces membrane curvature, while the SH3 domain recruits dynamin and other proteins to fission sites. Beyond CME, SNX9 participates in non-clathrin pathways, intracellular signaling, and cellular processes such as cell migration and division.
Recombinant SNX9 protein, typically expressed in *E. coli* or mammalian systems with affinity tags (e.g., GST, His-tag), is widely used to study its structural and functional properties *in vitro*. Purified SNX9 allows researchers to analyze its membrane-binding kinetics, oligomerization states, and interactions with cytoskeletal components or pathogens (e.g., viruses, bacteria) that hijack endocytic pathways. Studies using recombinant SNX9 have elucidated its phosphorylation-dependent regulation, role in disease contexts (e.g., cancer metastasis, neurological disorders), and potential as a therapeutic target. Its versatility in biochemical assays and cell-free systems continues to advance our understanding of membrane trafficking mechanisms.
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