| 纯度 | >95% SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDK2AP1 |
| Uniprot No | O14519-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-115aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAAHMPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYTQGT GNSQVPQSKY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAETERNA RS |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDK2AP1重组蛋白的3篇代表性文献，信息整理如下：
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1. **文献名称**：CDK2AP1 is a cell-cycle regulator essential for S phase entry and cell survival
**作者**：Kim et al.
**摘要**：该研究利用重组CDK2AP1蛋白揭示了其在细胞周期G1/S期转换中的关键作用，证明其通过调控Cyclin E-CDK2复合体活性促进DNA复制起始，并发现其缺失会导致细胞周期阻滞和凋亡。
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2. **文献名称**：Structural and functional characterization of recombinant CDK2AP1 in oral squamous cell carcinoma
**作者**：Shintani et al.
**摘要**：通过重组表达纯化CDK2AP1蛋白，结合体外实验发现其能抑制口腔鳞癌细胞增殖，机制涉及与p53通路互作并诱导细胞周期停滞，提示其作为潜在抑癌分子。
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3. **文献名称**：CDK2AP1 interacts with DNA polymerase alpha and is required for Okazaki fragment processing
**作者**：Dahl et al.
**摘要**：研究利用重组CDK2AP1蛋白进行互作实验，发现其通过与DNA聚合酶α结合参与DNA复制过程中的Okazaki片段加工，强调其在基因组稳定性维持中的功能。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或Web of Science核对最新研究。若需具体文献年份或期刊，可进一步补充关键词(如“重组表达技术”)进行筛选。
CDK2AP1 (Cyclin-Dependent Kinase 2-Associated Protein 1), also known as DOC-1. is a multifunctional protein implicated in cell cycle regulation, differentiation, and apoptosis. It was initially identified as a tumor suppressor due to its downregulation in various cancers, including oral squamous cell carcinoma and colorectal cancer. Structurally, it contains a conserved N-terminal domain critical for binding cyclin-dependent kinase 2 (CDK2), a key regulator of the G1/S phase transition. By inhibiting CDK2 activity, CDK2AP1 modulates cell cycle progression, ensuring genomic stability and preventing uncontrolled proliferation. Beyond its role in cancer, CDK2AP1 participates in epigenetic regulation, interacting with DNA methyltransferase 1 (DNMT1) to influence DNA methylation patterns during development.
Recombinant CDK2AP1 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional protein for research and therapeutic applications. This engineered form often includes affinity tags (e.g., His-tag) for simplified purification and detection. Researchers utilize recombinant CDK2AP1 to study its molecular interactions, particularly with CDK2 and DNMT1. and to explore its role in cellular pathways linked to cancer, stem cell differentiation, and tissue regeneration. In preclinical studies, it has shown potential as a therapeutic agent or biomarker for cancer treatment, given its ability to restore cell cycle control in malignant cells. Additionally, its involvement in epigenetic mechanisms makes it relevant for developmental biology and regenerative medicine. The production of recombinant CDK2AP1 enables high-throughput screening for drug candidates targeting CDK2 or DNMT1 pathways, bridging basic research and translational medicine.
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