| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPN10 |
| Uniprot No | Q9HC96 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-672aa |
| 氨基酸序列 | MRAGRGATPA RELFRDAAFP AADSSLFCDL STPLAQFRED ITWRRPQEIC ATPRLFPDDP REGQVKQGLL GDCWFLCACA ALQKSRHLLD QVIPPGQPSW ADQEYRGSFT CRIWQFGRWV EVTTDDRLPC LAGRLCFSRC QREDVFWLPL LEKVYAKVHG SYEHLWAGQV ADALVDLTGG LAERWNLKGV AGSGGQQDRP GRWEHRTCRQ LLHLKDQCLI SCCVLSPRAG ARELGEFHAF IVSDLRELQG QAGQCILLLR IQNPWGRRCW QGLWREGGEG WSQVDAAVAS ELLSQLQEGE FWVEEEEFLR EFDELTVGYP VTEAGHLQSL YTERLLCHTR ALPGAWVKGQ SAGGCRNNSG FPSNPKFWLR VSEPSEVYIA VLQRSRLHAA DWAGRARALV GDSHTSWSPA SIPGKHYQAV GLHLWKVEKR RVNLPRVLSM PPVAGTACHA YDREVHLRCE LSPGYYLAVP STFLKDAPGE FLLRVFSTGR VSLSAIRAVA KNTTPGAALP AGEWGTVQLR GSWRVGQTAG GSRNFASYPT NPCFPFSVPE GPGPRCVRIT LHQHCRPSDT EFHPIGFHIF QVPEGGRSQD APPLLLQEPL LSCVPHRYAQ EVSRLCLLPA GTYKVVPSTY LPDTEGAFTV TIATRIDRPS IHSQEMLGQF LQEVSIMAVM KT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPN10重组蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
1. **"Expression and Functional Analysis of Recombinant CAPN10 in Insulin Signaling"**
*作者：Tanaka A, et al. (2004)*
**摘要**：研究报道了在HEK293细胞中表达CAPN10重组蛋白，发现其通过调控IRS-1磷酸化影响胰岛素信号通路，可能与2型糖尿病发病机制相关。
2. **"Purification and Enzymatic Properties of Human CAPN10 Recombinant Protein"**
*作者：Lee S, Kim J. (2006)*
**摘要**：通过杆状病毒系统表达并纯化CAPN10重组蛋白，揭示其钙依赖性蛋白酶活性及底物特异性，为体外药物筛选提供模型。
3. **"Structural Characterization of CAPN10 Using Recombinant Protein Technology"**
*作者：Martinez R, et al. (2011)*
**摘要**：利用X射线晶体学解析CAPN10重组蛋白的三维结构，阐明其催化核心的构象变化及与钙离子结合的分子机制。
4. **"CAPN10 Recombinant Protein Modulates Mitochondrial Function in Pancreatic β-cells"**
*作者：Chen L, et al. (2018)*
**摘要**：通过原核表达系统获得CAPN10重组蛋白，发现其通过调节线粒体膜电位影响β细胞胰岛素分泌，提示其在糖尿病中的潜在作用。
**注意**：以上为模拟文献，实际研究需查阅PubMed、Web of Science等数据库获取准确信息。建议结合关键词“CAPN10 recombinant protein”或“calpain-10 expression”进行检索。
**Background of CAPN10 Recombinant Protein**
CAPN10 (Calpain 10) is a member of the calpain family of calcium-dependent cysteine proteases, which play critical roles in cellular processes such as signal transduction, apoptosis, and cytoskeletal remodeling. Unlike other calpains, CAPN10 lacks a classical calcium-binding domain but retains proteolytic activity, suggesting unique regulatory mechanisms. It gained prominence due to its association with type 2 diabetes mellitus (T2DM) through genome-wide studies linking *CAPN10* gene polymorphisms to increased disease susceptibility. This connection highlights its potential role in glucose metabolism and insulin secretion, though its exact molecular mechanisms remain under investigation.
Recombinant CAPN10 protein is produced via genetic engineering, often in *E. coli* or mammalian expression systems, to ensure high purity and functionality. This engineered protein enables researchers to study CAPN10’s structure, enzymatic activity, and interactions without interference from cellular contaminants. Key applications include elucidating its role in diabetes pathogenesis, exploring its involvement in mitochondrial function, and screening for inhibitors or modulators as potential therapeutics.
Studies using recombinant CAPN10 have revealed its ability to cleave substrates like cytoskeletal proteins and metabolic enzymes, influencing cell motility and energy homeostasis. Its atypical calcium sensitivity and tissue-specific expression patterns further underscore its regulatory complexity. Ongoing research aims to clarify how CAPN10 variants contribute to metabolic disorders and whether targeting this protease could offer novel treatment strategies.
Overall, CAPN10 recombinant protein serves as a vital tool for decoding the protein’s biological significance and translational potential in diabetes and related metabolic diseases.
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