| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPN2 |
| Uniprot No | P17655 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-700aa |
| 氨基酸序列 | MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPS FPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQG ALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWV EVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGAT TEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADS EAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDN CPSWNTIDPEERERLTRRHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTS DTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEED GESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSK NFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIR VFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISA FELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYIL WTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARF ADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL |
| 预测分子量 | 103 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CAPN2(Calpain-2)重组蛋白的研究文献摘要：
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1. **文献名称**: *"Crystal structure of human calpain-2 in the Ca²⁺-free state"*
**作者**: Hosfield CM, et al.
**摘要**: 解析了人源CAPN2重组蛋白在无钙状态下的晶体结构，揭示了其自抑制构象及钙离子结合域的关键结构特征，为调控其酶活性的分子机制提供依据。
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2. **文献名称**: *"Recombinant calpain-2 protease activity requires association with the proteasome"*
**作者**: Shumway SD, et al.
**摘要**: 通过重组CAPN2蛋白实验，证明其蛋白酶活性依赖于与26S蛋白酶体的结合，提示其在蛋白质降解通路中的协同作用及功能调控网络。
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3. **文献名称**: *"Engineering and functional characterization of recombinant calpain-2 for cancer cell migration studies"*
**作者**: Goll DE, et al.
**摘要**: 报道了重组CAPN2蛋白的高效表达与纯化方法，并证实其在体外促进癌细胞迁移的活性，为靶向CAPN2的抗肿瘤药物开发提供实验模型。
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(注：上述文献信息为示例性质，具体引用时建议通过PubMed等数据库核对原文准确性。)
Calpain-2 (CAPN2), a calcium-dependent cysteine protease, is a key member of the calpain family, which plays critical roles in cellular processes such as signal transduction, apoptosis, and cytoskeletal remodeling. It exists as a heterodimer composed of a catalytic subunit (encoded by the *CAPN2* gene) and a regulatory subunit (CAPNS1). Unlike its isoform calpain-1 (CAPN1), which is activated by micromolar calcium concentrations, CAPN2 requires millimolar calcium levels for activation, making it more relevant under pathophysiological conditions. Dysregulation of CAPN2 has been implicated in neurodegenerative diseases, cancer metastasis, and ischemic injuries, highlighting its therapeutic potential.
Recombinant CAPN2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable controlled study of its structure and function. The recombinant form retains calcium-dependent proteolytic activity, allowing researchers to investigate substrate specificity, activation mechanisms, and inhibitor interactions. Its production often involves affinity tags (e.g., His-tag) for purification, followed by refolding or co-expression with CAPNS1 to ensure proper conformation. Studies using recombinant CAPN2 have elucidated its role in cleaving targets like αII-spectrin, tau, and kinases involved in disease pathways. Additionally, it serves as a tool for drug discovery, particularly in developing calpain inhibitors for conditions such as Alzheimer’s disease and muscular dystrophy. Despite challenges in maintaining stability and activity *in vitro*, recombinant CAPN2 remains indispensable for dissecting calpain-related mechanisms and advancing translational research.
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