| 纯度 | >85 %SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDHOB |
| Uniprot No | P55287 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 54-617aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGWV WNQFFVIEEY TGPDPVLVGR LHSDIDSGDG NIKYILSGEG AGTIFVIDDK SGNIHATKTL DREERAQYTL MAQAVDRDTN RPLEPPSEFI VKVQDINDNP PEFLHETYHA NVPERSNVGT SVIQVTASDA DDPTYGNSAK LVYSILEGQP YFSVEAQTGI IRTALPNMDR EAKEEYHVVI QAKDMGGHMG GLSGTTKVMI TLTDVNDNPP KFPQSVYQMS VSEAAVPGEE VGRVKAKDPD IGENGLVTYN IVDGDGMESF EITTDYETQE GVIKLKKPVD FETKRAYSLK VEAANVHIDP KFISNGPFKD TVTVKIAVED ADEPPMFLAP SYIHEVQENA AAGTVVGRVH AKDPDAANSP IRYSIDRHTD LDRFFTINPE DGFIKTTKPL DREETAWLNI TVFAAEIHNR HQEAKVPVAI RVLDVNDNAP KFAAPYEGFI CESDQTKPLS NQPIVTISAD DKDDTANGPR FIFSLPPEII HNPNFTVRDN RDNTAGVYAR RGGFSRQKQD LYLLPIVISD GGIPPMSSTN TLTIKVCGCD VNGALLSCNA EAYILNAGLS T |
| 预测分子量 | 66 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDHOB重组蛋白的虚构参考文献示例(实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Structural and Functional Characterization of CDHOB Recombinant Protein in Cell Adhesion*
**作者**：Zhang L. et al.
**摘要**：本研究成功表达并纯化了CDHOB重组蛋白，通过X射线晶体学解析其三维结构，揭示其独特的钙离子结合域。功能实验表明，CDHOB在细胞间粘附和信号转导中起关键作用，尤其在神经元发育中具有潜在调控功能。
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2. **文献名称**：*CDHOB Recombinant Protein Expression in E. coli and Its Role in Cancer Metastasis*
**作者**：Smith J.R., Wang Y.
**摘要**：作者利用大肠杆菌系统高效表达CDHOB重组蛋白，并验证其与肿瘤转移的相关性。体外实验显示，CDHOB通过调控上皮-间质转化(EMT)抑制癌细胞侵袭，提示其作为潜在治疗靶点的价值。
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3. **文献名称**：*Development of a CDHOB-Based Biosensor for Detecting Cell Membrane Interactions*
**作者**：Kim S. et al.
**摘要**：本研究将CDHOB重组蛋白固定于生物传感器表面，实时监测其与配体分子的结合动力学。结果表明，CDHOB对特定脂质分子具有高亲和力，为膜生物学研究提供了新工具。
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4. **文献名称**：*Comparative Analysis of CDHOB Homologs in Vertebrate Evolution*
**作者**：Garcia M., Patel R.
**摘要**：通过比较基因组学分析多种脊椎动物的CDHOB同源蛋白，揭示了该蛋白在进化中的保守性差异。重组蛋白功能实验表明，哺乳动物CDHOB在组织形态发生中具有更复杂的功能调控网络。
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**注**：以上内容为示例性质，实际文献需根据具体研究方向在PubMed、Web of Science等平台检索。若CDHOB为特定领域术语，建议结合全称(如Cadherin-related protein CDHOB)或相关基因名(如CDH10/CDH12家族)扩展检索。
CDHOB (Cadherin-Related Oligomeric Binding protein) is a recombinant protein engineered to mimic the functional domains of classical cadherins, a family of calcium-dependent cell adhesion molecules critical for tissue morphogenesis, cell signaling, and intercellular communication. As a synthetic construct, CDHOB typically combines extracellular cadherin repeats responsible for homophilic binding with modified transmembrane or oligomerization domains to enhance stability and experimental utility. Its design leverages recombinant DNA technology, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications, particularly glycosylation, which influences its adhesion properties.
The protein has gained attention in structural biology for studying cadherin-mediated cell-cell interactions, which are pivotal in cancer metastasis, neuronal connectivity, and epithelial barrier maintenance. Researchers utilize CDHOB to dissect the mechanics of cadherin clustering, adhesion strength modulation, and cross-talk with intracellular signaling pathways like Wnt/β-catenin. Unlike native cadherins, CDHOB's engineered components allow controlled oligomerization, enabling precise investigation of adhesion mechanics under varying calcium concentrations or mechanical stresses.
Pharmaceutical applications include screening for cadherin-targeted therapeutics in diseases involving disrupted cell adhesion, such as invasive carcinomas or inflammatory disorders. However, challenges persist in replicating the full complexity of native cadherin interactions, particularly regarding tissue-specific isoforms and force-dependent conformational changes. Recent studies focus on optimizing CDHOB's modular design to incorporate fluorescent tags or force-sensing modules, expanding its utility in live-cell imaging and mechanobiology research.
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