| 纯度 | >85% SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDCP1 |
| Uniprot No | Q9H5V8-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343aa |
| 氨基酸序列 | MAGLNCGVSI ALLGVLLLGA ARLPRGAEAF EIALPRESNI TVLIKLGTPT LLAKPCYIVI SKRHITMLSI KSGERIVFTF SCQSPENHFV IEIQKNIDCM SGPCPFGEVQ LQPSTSLLPT LNRTFIWDVK AHKSIGLELQ FSIPRLRQIG PGESCPDGVT HSISGRIDAT VVRIGTFCSN GTVSRIKMQE GVKMALHLPW FHPRNVSGFS IANRSSIKRL CIIESVFEGE GSATLMSANY PEGFPEDELM TWQFVVPAHL RASVSFLNFN LSNCERKEER VEYYIPGSTT NPEVFKLEDK QPGNMAGNFN LSLQGCDQDA QSPGILRLQF QVLVQHPQNE SSE |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDCP1重组蛋白的3篇参考文献概览，包含文献名称、作者及摘要内容概括：
---
1. **文献名称**：*CDCP1 regulates the function of tumor-associated macrophages in breast cancer*
**作者**：Smith A, et al. (2020)
**摘要**：本研究利用重组CDCP1蛋白探究其在乳腺癌微环境中对巨噬细胞极化的调控机制。实验表明，CDCP1通过激活Src激酶信号通路促进肿瘤相关巨噬细胞(TAMs)的促癌表型，重组蛋白的体外实验验证了其与整合素受体的相互作用。
---
2. **文献名称**：*Expression and purification of recombinant human CDCP1 in mammalian cells for functional studies*
**作者**：Zhang Y, et al. (2018)
**摘要**：作者通过哺乳动物表达系统成功表达并纯化功能性重组人源CDCP1蛋白，优化了其糖基化修饰条件。该蛋白被用于研究CDCP1在细胞迁移中的作用，证实其通过结合膜表面蛋白酶调控细胞外基质重塑。
---
3. **文献名称**：*Structural insights into CDCP1-mediated signaling via its recombinant extracellular domain*
**作者**：Tanaka K, et al. (2016)
**摘要**：通过解析重组CDCP1胞外结构域的晶体结构，揭示了其与配体(如EGFR)结合的分子机制。研究为靶向CDCP1的癌症治疗策略提供了结构生物学基础。
---
**备注**：以上文献为示例性内容，实际文献需通过PubMed或Web of Science检索关键词“CDCP1 recombinant protein”或结合具体研究领域筛选。如需具体文献，建议补充研究背景(如疾病模型、实验方法等)。
CDCP1 (CUB domain-containing protein 1), also known as Trask (Transmembrane and associated with Src kinases), is a cell surface glycoprotein encoded by the *CDCP1* gene in humans. It belongs to the CUB (Complement C1r/C1s, Uegf, Bmp1) domain-containing protein family and is characterized by three extracellular CUB domains, a transmembrane region, and cytoplasmic tyrosine phosphorylation sites. Initially identified in 2000. CDCP1 is expressed in various epithelial tissues and plays roles in cell adhesion, migration, and signaling. Its dysregulation has been linked to cancer progression, particularly in metastasis, where it promotes tumor cell survival, invasion, and resistance to apoptosis.
Recombinant CDCP1 protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the protein for research. This tool enables studies on CDCP1's molecular interactions, such as its binding to extracellular matrix components (e.g., collagen) or intracellular signaling partners (e.g., Src kinases). Researchers use recombinant CDCP1 to investigate its role in activating pathways like PI3K/AKT and MAPK, which drive oncogenic behaviors. Additionally, it aids in developing therapeutic strategies, including monoclonal antibodies or small-molecule inhibitors targeting CDCP1 in cancers such as breast, lung, and colorectal carcinomas. Its soluble forms are also explored as biomarkers for disease monitoring. Despite progress, challenges remain in understanding context-dependent signaling and optimizing clinical applications.
×
