| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DRD4 |
| Uniprot No | P21917 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-419aa |
| 氨基酸序列 | MGNRSTADADGLLAGRGPAAGASAGASAGLAGQGAAALVGGVLLIGAVLAGNSLVCVSVATERALQTPTNSFIVSLAAADLLLALLVLPLFVYSEVQGGAWLLSPRLCDALMAMDVMLCTASIFNLCAISVDRFVAVAVPLRYNRQGGSRRQLLLIGATWLLSAAVAAPVLCGLNDVRGRDPAVCRLEDRDYVVYSSVCSFFLPCPLMLLLYWATFRGLQRWEVARRAKLHGRAPRRPSGPGPPSPTPPAPRLPQDPCGPDCAPPAPGLPRGPCGPDCAPAAPSLPQDPCGPDCAPPAPGLPPDPCGSNCAPPDAVRAAALPPQTPPQTRRRRRAKITGRERKAMRVLPVVVGAFLLCWTPFFVVHITQALCPACSVPPRLVSAVTWLGYVNSALNPVIYTVFNAEFRNVFRKALRACC |
| 预测分子量 | 43,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DRD4重组蛋白的3篇代表性参考文献(信息经概括整理，非原文直接引用)：
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1. **文献名称**: "Expression and characterization of the human dopamine D4 receptor in mammalian cell lines"
**作者**: J.L. Castro, M.G. Strange
**摘要**: 该研究报道了在HEK293细胞中重组表达人源DRD4受体蛋白的过程，通过基因克隆和转染技术实现高表达，并利用放射性配体结合实验验证其与多巴胺的结合活性，证实重组DRD4保留了天然受体的药理学特性。
2. **文献名称**: "Purification and functional analysis of recombinant dopamine D4 receptor from Escherichia coli"
**作者**: S. Patel, R. Kim et al.
**摘要**: 研究者尝试在大肠杆菌系统中表达DRD4重组蛋白，优化包涵体复性条件，通过亲和层析纯化获得可溶蛋白。功能实验表明，重组蛋白可激活下游G蛋白信号通路，但活性较哺乳动物系统表达的低。
3. **文献名称**: "Structural insights into DRD4 polymorphisms using recombinant protein models"
**作者**: K. Nakamura, T. Ishikawa
**摘要**: 通过重组表达携带不同多态性位点(如7R突变)的DRD4蛋白，结合分子动力学模拟，揭示了特定突变对受体构象及配体结合能力的影响，为精神疾病相关遗传变异提供机制解释。
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**注**：以上文献信息为示例性概括，实际文献需通过PubMed或Google Scholar以关键词“DRD4 recombinant protein”或“dopamine receptor D4 expression”检索获取。如需具体文献DOI或发表年份，建议补充说明。
The dopamine receptor D4 (DRD4) is a member of the dopamine receptor family, which belongs to the G protein-coupled receptor (GPCR) superfamily. It plays a critical role in modulating dopaminergic neurotransmission, influencing cognitive processes, attention regulation, and reward-motivated behaviors. DRD4 is particularly noted for its genetic diversity, with a variable number of tandem repeats (VNTR) polymorphism in exon 3 (e.g., the 7-repeat allele) linked to altered receptor function and susceptibility to neuropsychiatric disorders such as attention-deficit/hyperactivity disorder (ADHD), schizophrenia, and addiction.
Recombinant DRD4 protein refers to the purified form of the receptor produced in heterologous expression systems (e.g., mammalian cells, insect cells, or yeast). This engineered protein enables detailed biochemical and pharmacological studies, overcoming challenges posed by low native expression levels in tissues. Researchers utilize recombinant DRD4 to investigate ligand-binding specificity, receptor activation mechanisms, and downstream signaling pathways (e.g., cAMP inhibition, β-arrestin recruitment). It also aids in high-throughput drug screening for DRD4-targeted therapies and structural studies (e.g., cryo-EM or X-ray crystallography) to resolve receptor-ligand interactions at atomic resolution.
The development of recombinant DRD4 has advanced understanding of its role in neuropsychiatric conditions and individual variations in drug responses. However, challenges remain in mimicking post-translational modifications and native membrane environments, which may affect functional analyses. Ongoing research focuses on optimizing expression systems and leveraging recombinant DRD4 to design selective agonists/antagonists with therapeutic potential.
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