| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PARP4 |
| Uniprot No | Q9UKK3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 607-1046aa |
| 氨基酸序列 | SSTKAGLQDASGNLVPLEDVHIKGRIIDTVAQVIVFQTYTNKSHVPIEAKYIFPLDDKAAVCGFEAFINGKHIVGEIKEKEEAQQEYLEAVTQGHGAYLMSQDAPDVFTVSVGNLPPKAKVLIKITYITELSILGTVGVFFMPATVAPWQQDKALNENLQDTVEKICIKEIGTKQSFSLTMSIEMPYVIEFIFSDTHELKQKRTDCKAVISTMEGSSLDSSGFSLHIGLSAAYLPRMWVEKHPEKESEACMLVFQPDLDVDLPDLASESEVIICLDCSSSMEGVTFLQAKQIALHALSLVGEKQKVNIIQFGTGYKELFSYPKHITSNTMAAEFIMSATPTMGNTDFWKTLRYLSLLYPARGSRNILLVSDGHLQDESLTLQLVKRSRPHTRLFACGIGSTANRHVLRILSQCGAGVFEYFNAKSKHSWRKQIEDQMTRL |
| 预测分子量 | 55.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及PARP4重组蛋白研究的参考文献及其摘要概括：
1. **文献名称**: "Purification and Characterization of Recombinant PARP4/VPARP Protein from Insect Cells"
**作者**: Smith J, et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞表达系统成功表达并纯化了全长PARP4重组蛋白。通过酶活分析证实其具有ADP-核糖基转移酶活性，并揭示了其对微管蛋白的潜在修饰作用。
2. **文献名称**: "Structural Insights into PARP4 Domain Organization Using Recombinant Fragments"
**作者**: Lee S, et al.
**摘要**: 通过构建PARP4不同功能域的重组蛋白片段(包括VIT结构域和催化域)，利用X射线晶体学解析了其三维结构，阐明了PARP4与其他PARP家族成员的结构差异及底物结合特性。
3. **文献名称**: "Functional Analysis of PARP4 in DNA Repair Using Recombinant Knockout Models"
**作者**: Chen R, et al.
**摘要**: 通过重组蛋白互补实验，证明PARP4重组蛋白可恢复PARP4敲除细胞中DNA损伤修复缺陷，表明其在非同源末端连接(NHEJ)修复通路中的非冗余作用。
注：以上文献为示例性内容，实际文献需通过PubMed/Google Scholar以关键词"PARP4 recombinant"或"vPARP purification"检索获取。若需具体文献，建议补充研究领域(如癌症、DNA修复等)以精准筛选。
**Background of PARP4 Recombinant Protein**
PARP4 (Poly(ADP-ribose) polymerase 4), also known as ADP-ribosyltransferase diphtheria toxin-like 4 (ARTD4), is a member of the PARP superfamily, which plays critical roles in DNA repair, genomic stability, and cellular stress responses. Unlike its well-studied relatives PARP1 and PARP2. PARP4 is a less characterized enzyme but shares the conserved catalytic domain responsible for transferring ADP-ribose units to target proteins. PARP4 is distinguished by its large molecular weight (~200 kDa) and unique structural features, including multiple N-terminal macrodomains and a C-terminal catalytic domain, which suggest specialized functions in protein-protein interactions and signal transduction.
Recombinant PARP4 protein is engineered via molecular cloning, typically expressed in systems like *E. coli* or mammalian cell lines, to ensure proper post-translational modifications. This purified protein retains enzymatic activity and is widely used to study PARP4's biological roles, such as its involvement in mitotic regulation, immune response modulation, and tumor suppression. Emerging evidence links PARP4 to cancer progression, particularly in leukemia and breast cancer, where it may interact with BRCA1 or regulate double-strand break repair pathways.
In drug discovery, recombinant PARP4 serves as a tool to screen inhibitors targeting PARP family enzymes, especially for cancers with BRCA mutations. Its study also contributes to understanding PARP isoform-specific functions and developing next-generation therapies beyond PARP1/2 inhibitors. However, the full scope of PARP4's physiological and pathological roles remains under investigation, highlighting the importance of recombinant protein-based research in unraveling its complexity.
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