| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PARN |
| Uniprot No | O95453 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639aa |
| 氨基酸序列 | MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEERQLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQRKLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANSGKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELEKRLKETPFNPPKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSFLSPPKIHVSARSKLIEPFFNKLFLMRVMDIPYLNLEGPDLQPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQVKIAVNTSKYAESYRIQTYAEYMGRKQEEKQIKRKWTEDSWKEADSKRLNPQCIPYTLQNHYYRNNSFTAPSTVGKRNLSPSQEEAGLEDGVSGEISDTELEQTDSCAEPLSEGRKKAKKLKRMKKELSPAGSISKNSPATLFEVPDTW |
| 预测分子量 | 75.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于PARN(Poly(A)-specific ribonuclease)重组蛋白的假想参考文献示例(仅供学术写作参考，非真实文献)：
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1. **标题**: "Recombinant Human PARN: Expression, Purification, and Enzymatic Characterization"
**作者**: Smith J, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了重组人源PARN蛋白，优化了其可溶性表达条件。通过体外酶活实验验证了重组PARN对poly(A) RNA的特异性切割能力，并发现镁离子浓度对其活性有显著影响。
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2. **标题**: "PARN Regulates Telomerase RNA Maturation in Stem Cells"
**作者**: Chen L, et al.
**摘要**: 利用重组PARN蛋白研究其在胚胎干细胞中的作用，发现PARN通过剪切TERC RNA的poly(A)尾端调控端粒酶RNA的成熟，揭示了PARN缺陷导致端粒缩短及相关疾病的分子机制。
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3. **标题**: "Structural Insights into PARN Deadenylase by Cryo-EM"
**作者**: Gupta R, et al.
**摘要**: 通过冷冻电镜解析了重组PARN蛋白的三维结构，揭示了其催化结构域与RNA结合的关键氨基酸残基，为开发靶向PARN的小分子抑制剂提供了结构基础。
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注：以上文献为模拟内容，实际研究中需检索真实数据库(如PubMed、Google Scholar)获取。
**Background of PARN Recombinant Protein**
Poly(A)-specific ribonuclease (PARN), also known as deadenylating ribonuclease, is a key enzyme involved in post-transcriptional regulation of gene expression. It catalyzes the removal of poly(A) tails from messenger RNA (mRNA), a critical step in controlling mRNA stability, translation efficiency, and degradation. By shortening the poly(A) tail, PARN contributes to the regulation of mRNA turnover, ensuring timely protein synthesis and cellular homeostasis. This enzyme is particularly important during early development, stress responses, and in maintaining the integrity of RNA processing pathways.
PARN is a member of the DEDD (Asp-Glu-Asp-Asp) superfamily of nucleases and functions as a 3’-to-5’ exoribonuclease. Its structure includes a catalytic domain, an R3H RNA-binding motif, and a RNA recognition motif (RRM), which collectively enable substrate specificity and efficient deadenylation. Unlike other deadenylases, PARN exhibits a strong preference for longer poly(A) tails, making it a unique player in mRNA decay and quality control mechanisms.
Recombinant PARN protein is produced through heterologous expression systems, such as *E. coli* or eukaryotic cell cultures, enabling large-scale purification for biochemical and functional studies. Researchers utilize recombinant PARN to investigate its enzymatic kinetics, structural properties, and interactions with regulatory factors like the cap-binding complex or microRNAs. Dysregulation of PARN has been linked to diseases such as cancer, neurodegeneration, and developmental disorders, underscoring its therapeutic potential. For instance, reduced PARN activity is associated with mRNA stabilization in certain cancers, while mutations are implicated in telomere-related pathologies.
Recent studies also explore PARN’s role in non-coding RNA metabolism and its crosstalk with other RNA decay pathways. Recombinant PARN serves as a vital tool for drug discovery, aiming to modulate its activity for therapeutic interventions. Overall, PARN recombinant protein remains a focal point in understanding RNA biology and developing targeted treatments for RNA-related diseases.
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