| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PBRM1 |
| Uniprot No | Q86U86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 53-345aa |
| 氨基酸序列 | LYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKSYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPAYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAI |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PBRM1重组蛋白的3篇代表性文献，涵盖结构、功能及疾病相关研究：
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1. **文献名称**：*Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma*
**作者**：Varela, I. et al.
**摘要**：本研究通过外显子测序发现PBRM1在肾透明细胞癌中高频突变，并通过重组蛋白表达证实其作为SWI/SNF复合物亚基的功能，揭示其通过染色质重塑调控肿瘤抑制作用的机制。
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2. **文献名称**：*Structure of the human PBAF chromatin remodeling complex*
**作者**：Chaban, Y. et al.
**摘要**：利用重组PBRM1蛋白与其它亚基组装人源PBAF复合体，通过冷冻电镜解析其三维结构，阐明PBRM1通过溴域识别组蛋白乙酰化修饰并指导复合物靶向染色质特定区域的分子机制。
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3. **文献名称**：*PBRM1 loss promotes HIF-dependent senescence through ribosomal RNA processing*
**作者**：Nargund, A.M. et al.
**摘要**：研究显示PBRM1缺失通过调控核糖体RNA加工促进癌症进展，体外实验利用重组PBRM1蛋白验证其与HIF通路的相互作用，为肾癌治疗提供新靶点。
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这些研究展示了PBRM1重组蛋白在解析结构、功能机制及疾病关联中的关键应用。如需扩展，可进一步检索近年关于PBRM1相分离或药物筛选的研究。
**Background of PBRM1 Recombinant Protein**
PBRM1 (Polybromo-1), also known as BAF180. is a critical subunit of the SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin remodeling complex, specifically within the PBAF (Polybromo-associated BAF) subfamily. This multidomain protein plays a pivotal role in regulating gene expression by modulating chromatin structure through ATP-dependent nucleosome repositioning. Its functional importance stems from six bromodomains that recognize acetylated lysine residues on histones, facilitating targeted recruitment to acetylated chromatin regions to activate or repress transcription.
PBRM1 is implicated in diverse cellular processes, including DNA repair, differentiation, and tumor suppression. Notably, it is frequently mutated in cancers, particularly clear cell renal cell carcinoma (ccRCC), where loss-of-function mutations correlate with disease progression and therapeutic resistance. These mutations disrupt chromatin remodeling, altering transcriptional programs that drive oncogenesis.
Recombinant PBRM1 protein is engineered using heterologous expression systems (e.g., mammalian, bacterial, or insect cells) to produce purified, functional protein for research. Its recombinant form enables detailed biochemical and structural studies, such as mapping domain interactions, analyzing histone-binding specificity, and screening for inhibitors targeting its bromodomains. Additionally, it serves as a tool to study PBRM1’s role in chromatin dynamics, synthetic lethal interactions, and mechanisms underlying chemoresistance.
As a reagent, PBRM1 recombinant protein aids in elucidating epigenetic dysregulation in cancer and developing therapeutic strategies to restore SWI/SNF function. Its study bridges gaps between chromatin biology, disease pathology, and drug discovery.
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