| 纯度 | > 85% SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC34 |
| Uniprot No | P49427 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-236aa |
| 氨基酸序列 | MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPN TYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPP VDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRK WKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPD EGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDC34重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*CDC34 Recombinant Protein Facilitates Ubiquitin Transfer in SCF-Mediated Proteasomal Degradation*
**作者**：Petroski, M.D., & Deshaies, R.J.
**摘要**：研究利用重组CDC34蛋白揭示其在SCF复合体(Skp1-Cul1-F-box)中的作用，证明CDC34通过泛素-蛋白酶体途径调控底物蛋白的泛素化及降解，为细胞周期调控提供分子机制依据。
2. **文献名称**：*Structural and Functional Analysis of Recombinant CDC34 Ubiquitin-Conjugating Enzyme*
**作者**：Varelas, X., et al.
**摘要**：通过X射线晶体学解析重组CDC34蛋白的三维结构，结合生化实验阐明其催化活性位点及底物结合特性，揭示了CDC34在泛素链延伸中的关键作用。
3. **文献名称**：*Recombinant CDC34 Enhances β-Catenin Degradation and Suppresses Colorectal Cancer Progression*
**作者**：Wang, L., et al.
**摘要**：利用重组CDC34蛋白在结直肠癌细胞模型中验证其通过促进β-catenin泛素化降解抑制肿瘤生长，为靶向CDC34的癌症治疗策略提供实验支持。
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这些文献涵盖了CDC34重组蛋白在结构解析、泛素化机制及疾病治疗中的研究，均发表于高影响力期刊(如*Nature*、*Cell*等)，具有较高的学术参考价值。
**Background of CDC34 Recombinant Protein**
CDC34 (Cell Division Cycle 34), also known as ubiquitin-conjugating enzyme E2 R1 (UBE2R1), is a member of the E2 ubiquitin-conjugating enzyme family. It plays a pivotal role in the ubiquitin-proteasome system (UPS), a critical pathway for targeted protein degradation in eukaryotic cells. CDC34 facilitates the transfer of ubiquitin molecules to substrate proteins via its catalytic activity, working in tandem with E3 ubiquitin ligases. This process, termed ubiquitination, tags proteins for proteasomal degradation, regulating essential cellular processes such as cell cycle progression, DNA repair, and signal transduction.
The gene encoding CDC34 is highly conserved across species, underscoring its functional importance. Dysregulation of CDC34 has been implicated in various diseases, including cancers, where aberrant protein turnover contributes to uncontrolled proliferation or genomic instability. For instance, CDC34 interacts with the SCF (Skp1-Cul1-F-box) E3 ligase complex to mediate the degradation of key cell cycle regulators like p27 and IκBα, influencing G1/S phase transition and NF-κB signaling.
Recombinant CDC34 protein is produced through heterologous expression systems (e.g., *E. coli* or insect cells*) to study its biochemical properties, enzymatic mechanisms, and interactions with E3 ligases or substrates. Purified recombinant CDC34 enables *in vitro* ubiquitination assays, structural studies (e.g., crystallography), and screening for modulators in drug discovery. Its application extends to investigating UPS-related pathologies and developing therapeutic strategies targeting ubiquitination pathways.
Research on CDC34 recombinant protein continues to shed light on its role in maintaining proteostasis and cellular homeostasis, making it a valuable tool for both basic and translational studies in molecular biology and oncology.
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