| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPYSL3 |
| Uniprot No | Q14195 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-570aa |
| 氨基酸序列 | MSYQGKKNIPRITSDRLLIKGGRIVNDDQSFYADIYMEDGLIKQIGDNLI VPGGVKTIEANGKMVIPGGIDVHTHFQMPYKGMTTVDDFFQGTKAALAGG TTMIIDHVVPEPESSLTEAYEKWREWADGKSCCDYALHVDITHWNDSVKQ EVQNLIKDKGVNSFMVYMAYKDLYQVSNTELYEIFTCLGELGAIAQVHAE NGDIIAQEQTRMLEMGITGPEGHVLSRPEELEAEAVFRAITIASQTNCPL YVTKVMSKSAADLISQARKKGNVVFGEPITASLGIDGTHYWSKNWAKAAA FVTSPPLSPDPTTPDYINSLLASGDLQLSGSAHCTFSTAQKAIGKDNFTA IPEGTNGVEERMSVIWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGR ISVGSDSDLVIWDPDAVKIVSAKNHQSAAEYNIFEGMELRGAPLVVICQG KIMLEDGNLHVTQGAGRFIPCSPFSDYVYKRIKARRKMADLHAVPRGMYD GPVFDLTTTPKGGTPAGSARGSPTRPNPPVRNLHQSGFSLSGTQVDEGVR SASKRIVAPPGGRSNITSLS |
| 预测分子量 | 88 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPYSL3重组蛋白的3篇参考文献及其摘要概括(文献为示例性内容，实际引用需核实)：
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1. **文献名称**：*Recombinant DPYSL3 regulates axonal growth through interaction with Sema3A*
**作者**：Smith J, et al.
**摘要**：研究通过表达纯化重组DPYSL3蛋白，揭示了其与Sema3A信号通路的相互作用，调控神经元轴突生长，为神经再生机制提供了新见解。
2. **文献名称**：*Expression and purification of CRMP3/DPYSL3 in E. coli for structural analysis*
**作者**：Li H, et al.
**摘要**：成功在大肠杆菌中高效表达并纯化重组DPYSL3蛋白，通过X射线晶体学解析其三维结构，为后续功能研究与药物开发奠定基础。
3. **文献名称**：*DPYSL3 modulates cancer cell migration via RhoA signaling pathway*
**作者**：Chen W, et al.
**摘要**：利用重组DPYSL3蛋白处理多种癌细胞系，发现其通过抑制RhoA通路显著降低细胞迁移能力，提示其在癌症转移中的潜在治疗价值。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“DPYSL3 recombinant protein”或“CRMP3 expression”获取最新研究。
**Background of DPYSL3 Recombinant Protein**
DPYSL3 (Dihydropyrimidinase-Like 3), also known as CRMP3 (Collapsin Response Mediator Protein 3), is a cytosolic phosphoprotein belonging to the dihydropyrimidinase-related protein family. It is primarily expressed in the nervous system and plays a critical role in neuronal development, including axon guidance, dendritic outgrowth, and synaptic plasticity. DPYSL3 interacts with tubulin heterodimers to regulate microtubule dynamics, influencing cytoskeletal organization and cellular motility. Its activity is modulated by phosphorylation, which alters its ability to bind partners like kinases or scaffolding proteins, thereby fine-tuning signaling pathways such as Semaphorin and Rho GTPase cascades.
Recombinant DPYSL3 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research applications. By omitting native post-translational modifications or introducing specific mutations, recombinant forms allow targeted studies of DPYSL3’s structure-function relationships. This protein is widely used to investigate mechanisms underlying neurodevelopmental disorders, neurodegenerative diseases (e.g., Alzheimer’s), and cancer, where dysregulated DPYSL3 expression correlates with tumor metastasis and poor prognosis.
In drug discovery, recombinant DPYSL3 serves as a tool for screening molecules that modulate its interaction with cytoskeletal components or signaling intermediates. Additionally, it aids in antibody development for diagnostic assays, given its potential as a biomarker in neurological and oncological contexts. Studies leveraging recombinant DPYSL3 continue to unravel its dual roles in neuroprotection and disease progression, highlighting its therapeutic and diagnostic relevance.
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