关键信息
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基因名
TBC1D14
- 应用
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别名
FLJ32400; KIAA1322; TBC1 domain family member 14; TBC14_HUMAN; Tbc1d14
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9P2M4
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表达区间
1-693 aa
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氨基酸序列
MTDGKLSTST NGVAFMGILD GRPGNPLQNL QHVNLKAPRL LSAPEYGPKL KLRALEDRHS LQSVDSGIPT LEIGNPEPVP CSAVHVRRKQ SDSDLIPERA FQSACALPSC APPAPSSTER EQSVRKSSTF PRTGYDSVKL YSPTSKALTR SDDVSVCSVS SLGTELSTTL SVSNEDILDL VVTSSSSAIV TLENDDDPQF TNVTLSSIKE TRGLHQQDCV HEAEEGSKLK ILGPFSNFFA RNLLARKQSA RLDKHNDLGW KLFGKAPLRE NAQKDSKRIQ KEYEDKAGRP SKPPSPKQNV RKNLDFEPLS TTALILEDRP ANLPAKPAEE AQKHRQQYEE MVVQAKKREL KEAQRRKKQL EERCRVEESI GNAVLTWNNE ILPNWETMWC SRKVRDLWWQ GIPPSVRGKV WSLAIGNELN ITHELFDICL ARAKERWRSL STGGSEVENE DAGFSAADRE ASLELIKLDI SRTFPNLCIF QQGGPYHDML HSILGAYTCY RPDVGYVQGM SFIAAVLILN LDTADAFIAF SNLLNKPCQM AFFRVDHGLM LTYFAAFEVF FEENLPKLFA HFKKNNLTPD IYLIDWIFTL YSKSLPLDLA CRIWDVFCRD GEEFLFRTAL GILKLFEDIL TKMDFIHMAQ FLTRLPEDLP AEELFASIAT IQMQSRNKKW AQVLTALQKD SREMEKGSPS LRH
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分子量
78.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
TBC1D14 is a member of the TBC (Tre-2/Bub2/Cdc16) domain family, which is implicated in the regulation of intracellular vesicle transport and signaling pathways. Research on TBC1D14 has gained momentum due to its potential role in cellular processes such as autophagy and insulin signaling, which are crucial for maintaining cellular homeostasis. Dysregulation of TBC1D14 has been linked to various diseases, including obesity and diabetes, making it an important target for understanding metabolic disorders. The generation of recombinant TBC1D14 protein facilitates extensive biochemical and structural studies that can elucidate its functional mechanisms. These studies have the potential to reveal how TBC1D14 interacts with other proteins and contributes to cellular processes, further highlighting its significance in health and disease. The insights gained from this research may pave the way for novel therapeutic strategies aimed at modulating TBC1D14 activity to combat related metabolic diseases. Overall, the investigation of TBC1D14 through recombinant protein studies serves as a vital stepping stone in deciphering its biological roles and therapeutic implications.
