| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPEP3 |
| Uniprot No | Q9H4B8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-463aa |
| 氨基酸序列 | AETTPGAPRALSTLGSPSLFTTPGVPSALTTPGLTTPGTPKTLDLRGRAQALMRSFPLVDGHNDLPQVLRQRYKNVLQDVNLRNFSHGQTSLDRLRDGLVGAQFWSASVSCQSQDQTAVRLALEQIDLIHRMCASYSELELVTSAEGLNSSQKLACLIGVEGGHSLDSSLSVLRSFYVLGVRYLTLTFTCSTPWAESSTKFRHHMYTNVSGLTSFGEKVVEELNRLGMMIDLSYASDTLIRRVLEVSQAPVIFSHSAARAVCDNLLNVPDDILQLLKKNGGIVMVTLSMGVLQCNLLANVSTVADHFDHIRAVIGSEFIGIGGNYDGTGRFPQGLEDVSTYPVLIEELLSRSWSEEELQGVLRGNLLRVFRQVEKVREESRAQSPVEAEFPYGQLSTSCHSHLVPQNGHQATHLEVTKQPTNRVPWRS |
| 预测分子量 | 48.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于DPEP3重组蛋白的参考文献示例(内容基于公开研究领域知识总结，具体文献信息请通过学术数据库验证)：
1. **《DPEP3 as a novel biomarker for testicular germ cell tumors》**
作者：Smith A, et al.
摘要：研究证实DPEP3在睾丸生殖细胞肿瘤中特异性高表达，通过重组蛋白技术获得DPEP3胞外段，证明其可作为血清诊断标志物，并探讨了其在肿瘤靶向治疗中的潜力。
2. **《Recombinant DPEP3 expression in E. coli and enzymatic characterization》**
作者：Zhang L, et al.
摘要：报道了利用大肠杆菌系统高效表达DPEP3重组蛋白的优化方法，分析其金属离子依赖性二肽酶活性，并解析了底物特异性，为后续功能研究提供基础。
3. **《DPEP3 knockdown inhibits prostate cancer cell invasion via EMT pathway modulation》**
作者：Wang Y, et al.
摘要：通过构建DPEP3重组蛋白进行体外功能实验，发现其通过调控上皮间质转化(EMT)影响前列腺癌细胞迁移，提示其在肿瘤转移中的分子机制。
4. **《Structural analysis of human DPEP3 by X-ray crystallography》**
作者：Tanaka K, et al.
摘要：首次解析了重组DPEP3蛋白的晶体结构，揭示了其活性中心的关键氨基酸残基及与锌离子结合模式，为设计特异性抑制剂奠定结构基础。
注：以上为模拟文献摘要，实际文献需通过PubMed/Google Scholar等平台以“DPEP3”或“dipeptidase 3”为关键词检索确认。
**Background of DPEP3 Recombinant Protein**
Dipeptidase 3 (DPEP3), a member of the membrane-bound dipeptidase family, is a zinc-dependent metalloprotease encoded by the *DPEP3* gene. It is primarily expressed in the testis and has garnered attention for its potential roles in male reproductive biology, particularly in sperm maturation and function. Structurally, DPEP3 shares homology with other dipeptidases (e.g., DPEP1 and DPEP2), featuring conserved catalytic domains essential for hydrolyzing dipeptides, though its specific substrates and physiological functions remain less characterized compared to its homologs.
Recombinant DPEP3 protein is engineered using biotechnological platforms, such as mammalian or bacterial expression systems, to produce purified, functional protein for research applications. Its recombinant form enables detailed studies on enzymatic activity, substrate specificity, and interactions with biological molecules. Notably, DPEP3 has been implicated in cancer research, with studies suggesting its overexpression in certain malignancies, including testicular germ cell tumors, where it may serve as a biomarker or therapeutic target.
The development of recombinant DPEP3 also supports antibody production, structural analysis (e.g., X-ray crystallography), and in vitro assays to elucidate its role in cellular processes. Challenges include optimizing expression yields and maintaining enzymatic fidelity during purification. Current research aims to clarify DPEP3's involvement in fertility disorders and cancer progression, highlighting its dual significance in basic science and translational medicine. Overall, DPEP3 recombinant protein serves as a critical tool for unraveling the biological and pathological pathways associated with this understudied enzyme.
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