| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGAT1 |
| Uniprot No | O75907 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 240-488aa |
| 氨基酸序列 | TVSYPDNLTYRDLYYFLFAPTLCYELNFPRSPRIRKRFLLRRILEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIWLIFFYWLFHSCLNAVAELMQFGDREFYRDWWNSESVTYFWQNWNIPVHKWCIRHFYKPMLRRGSSKWMARTGVFLASAFFHEYLVSVPLRMFRLWAFTGMMAQIPLAWFVGRFFQGNYGNAAVWLSLIIGQPIAVLMYVHDYYVLNYEAPAAEA |
| 预测分子量 | 37.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DGAT1重组蛋白的3篇参考文献示例，涵盖其功能研究、结构分析和应用开发：
1. **"Cloning of DGAT1. a novel mammalian triglyceride synthesis enzyme"**
*Authors: Cases, S., Smith, S.J., Zheng, Y.W. et al. (1998)*
**摘要**：该研究首次克隆了哺乳动物DGAT1基因，并在昆虫细胞中表达重组DGAT1蛋白，证实其能够催化二酰基甘油(DAG)转化为甘油三酯(TAG)，奠定了DGAT1在脂代谢中的关键作用。
2. **"Membrane topology and catalytic domains of DGAT1: Insights from recombinant protein analysis"**
*Authors: McFie, P.J., Banaszak, L.J., Stone, S.J. (2010)*
**摘要**：通过在大肠杆菌和哺乳动物细胞中表达重组DGAT1.结合酶活性实验，揭示了DGAT1的膜结合特性及催化结构域，发现其活性依赖特定的膜微环境。
3. **"Structural basis for catalysis and substrate specificity of human DGAT1"**
*Authors: Sui, X., Wang, Y., Kossiakoff, A.A. et al. (2020)*
**摘要**：利用冷冻电镜解析了重组人源DGAT1的高分辨率结构，阐明了其底物识别和催化机制，为靶向药物设计提供了结构基础。
4. **"A high-throughput assay for DGAT1 inhibitor screening using purified recombinant enzyme"**
*Authors: Smith, J.L., Lee, E.K., Waggoner, J.R. (2015)*
**摘要**：开发了一种基于重组DGAT1蛋白的体外高通量筛选平台，用于快速鉴定选择性抑制剂，助力代谢性疾病治疗研究。
*注：上述文献信息为示例性质，实际引用时需核实具体来源及细节。*
**Background of DGAT1 Recombinant Protein**
Diacylglycerol O-acyltransferase 1 (DGAT1) is a key enzyme in lipid metabolism, primarily catalyzing the final step of triglyceride synthesis by transferring a fatty acyl group from acyl-CoA to diacylglycerol. Encoded by the *DGAT1* gene in humans, this endoplasmic reticulum-resident membrane protein belongs to the membrane-bound O-acyltransferase (MBOAT) family. DGAT1 plays a critical role in dietary fat absorption, lipid storage, and lipoprotein assembly, making it a focal point in metabolic disease research, including obesity, diabetes, and cardiovascular disorders.
Recombinant DGAT1 protein is engineered through heterologous expression systems (e.g., bacterial, mammalian, or insect cells) to study its structure, function, and regulatory mechanisms. Its production enables in vitro analysis of enzymatic activity, substrate specificity, and inhibitor screening. DGAT1’s hydrophobic nature and complex membrane-associated structure pose challenges in purification, often requiring detergent solubilization and affinity tag strategies.
Pharmaceutically, DGAT1 is a therapeutic target; its inhibition reduces triglyceride accumulation, offering potential treatments for metabolic syndromes. Recombinant DGAT1 facilitates drug discovery by enabling high-throughput assays and structural studies (e.g., crystallography or cryo-EM) to elucidate inhibitor binding sites. Additionally, mutations in *DGAT1* are linked to congenital disorders like diarrhea-predominant enteropathy, underscoring its physiological importance.
Research on DGAT1 recombinant protein also explores its role in cellular lipid droplet formation and interactions with other lipid-metabolizing enzymes. Despite progress, challenges remain in mimicking its native membrane environment and post-translational modifications. Ongoing efforts aim to refine expression systems and functional assays to advance therapeutic development and mechanistic understanding.
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