| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGKg |
| Uniprot No | P49619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 氨基酸序列 | MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQYDPHEPISYDVFKLFMRAYLEVDLPQPLSTHLFLAFSQKPRHETSDHPTEGASNSEANSADTNIQNADNATKADEACAPDTESNMAEKQAPAEDQVAATPLEPPVPRSSSSESPVVYLKDVVCYLSLLETGRPQDKLEFMFRLYDSDENGLLDQAEMDCIVNQMLHIAQYLEWDPTELRPILKEMLQGMDYDRDGFVSLQEWVHGGMTTIPL |
| 预测分子量 | 44.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DGKγ(二酰甘油激酶γ亚型)重组蛋白的3篇参考文献示例(文献信息为虚构，仅供格式参考)：
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1. **文献名称**：*Expression and Purification of Recombinant Human DGKγ in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：该研究报道了通过大肠杆菌表达系统成功制备重组人源DGKγ蛋白，优化了纯化步骤并验证其激酶活性，为体外酶学研究提供了可靠工具。
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2. **文献名称**：*Structural Insights into DGKγ Catalytic Mechanism Using Cryo-EM*
**作者**：Zhang Y, et al.
**摘要**：利用冷冻电镜解析了重组DGKγ蛋白的三维结构，揭示了其与底物二酰甘油(DAG)结合的活性位点，阐明了其催化DAG磷酸化的分子机制。
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3. **文献名称**：*DGKγ Overexpression Modulates T Cell Signaling in Cancer*
**作者**：Lee H, et al.
**摘要**：通过哺乳动物细胞表达重组DGKγ，发现其过表达抑制T细胞受体信号通路，提示DGKγ可能通过调控脂质代谢影响肿瘤免疫微环境。
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注：以上文献信息为示例，实际研究中建议通过PubMed、Web of Science等数据库检索真实文献。
**Background of DGKγ (Diacylglycerol Kinase Gamma) Recombinant Protein**
DGKγ, a member of the diacylglycerol kinase (DGK) family, is an enzyme that phosphorylates diacylglycerol (DAG) to produce phosphatidic acid (PA), playing a critical role in lipid signaling and cellular homeostasis. This isoform, encoded by the *DGKG* gene in humans, is distinguished by its unique structural features, including a pleckstrin homology (PH) domain, a cysteine-rich C1 domain, and a catalytic domain. DGKγ is predominantly expressed in the brain, retina, and immune cells, where it regulates key processes such as membrane trafficking, receptor signaling, and cell proliferation.
Recombinant DGKγ protein is engineered through molecular cloning and expressed in heterologous systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties and physiological roles. Its production enables detailed analysis of enzymatic activity, substrate specificity, and interactions with signaling molecules like protein kinase C (PKC) and Rho GTPases. DGKγ’s ability to modulate DAG and PA levels positions it as a pivotal regulator of pathways implicated in cancer, inflammation, and neurological disorders. For instance, dysregulated DGKγ activity has been linked to impaired T-cell function and neurodegenerative conditions.
Researchers utilize recombinant DGKγ to explore its therapeutic potential, including targeting lipid metabolism in diseases such as diabetes and Alzheimer’s. Structural studies of the recombinant protein also aid in designing inhibitors or activators to fine-tune its activity. Overall, DGKγ recombinant protein serves as a vital tool for dissecting lipid-mediated signaling networks and developing novel treatment strategies.
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