| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGKe |
| Uniprot No | P52429 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567aa |
| 氨基酸序列 | MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVFITFWCSLQRSRRQLH RRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLR KADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPK LCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRK DKKTDYEVLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVF DVTKTPPIKALQLCTLLPYYSARVLVCGGDGTVGWVLDAVDDMKIKGQEK YIPQVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRW KVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSR ILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVALPSLEGIIVLNIG YWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFR IGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHAMMLYFSGEQT DDDISSTSDQEDIKATE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DGKε(二酰基甘油激酶ε型)重组蛋白的3篇代表性文献的简要信息，供参考：
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1. **文献名称**: *"Cloning and characterization of DGKε, a novel diacylglycerol kinase"*
**作者**: Sasaki J, et al.
**期刊/年份**: Journal of Biological Chemistry, 2003
**摘要**: 本研究首次克隆并鉴定了DGKε亚型，发现其通过特异性催化二酰基甘油(DAG)生成磷脂酸(PA)，在脂质代谢中具有独特功能。重组DGKε蛋白在体外实验中表现出对含花生四烯酸DAG底物的高选择性。
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2. **文献名称**: *"Regulation of DGKε activity and its role in lipid-mediated signaling"*
**作者**: Shulga YV, et al.
**期刊/年份**: Cellular Signalling, 2011
**摘要**: 通过重组DGKε蛋白的功能研究，揭示了其活性受磷酸化和膜定位调控的机制，并证明其在抑制DAG-PKC信号通路、维持细胞膜脂质稳态中的关键作用。
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3. **文献名称**: *"DGKε dysfunction in metabolic disorders: Insights from recombinant protein studies"*
**作者**: Merida I, et al.
**期刊/年份**: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2015
**摘要**: 利用重组DGKε蛋白模型，发现其活性异常与胰岛素抵抗和脂代谢疾病相关，提示DGKε可能成为代谢综合征的潜在治疗靶点。
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**注意**：以上文献信息为示例性质，具体引用时请通过PubMed、Google Scholar等平台核对原文准确性。实际研究中，可结合关键词“DGKε recombinant protein”“diacylglycerol kinase epsilon”进一步检索最新进展。
DGKe (diacylglycerol kinase epsilon) is a lipid-metabolizing enzyme belonging to the diacylglycerol kinase (DGK) family, which catalyzes the conversion of diacylglycerol (DAG) to phosphatidic acid (PA). These lipid mediators play critical roles in cellular signaling, membrane dynamics, and metabolic regulation. DGKe was first identified in the late 1990s as a unique isoform distinguished by its structural features, including a pleckstrin homology (PH) domain, ankyrin repeats, and a catalytic domain conserved across DGKs. Unlike other DGK isoforms, DGKe exhibits tissue-specific expression, predominantly in the brain, immune cells, and testis, suggesting specialized roles in neuronal function, immune response, and reproduction.
Research has highlighted DGKe’s regulatory role in balancing DAG and PA levels, thereby modulating downstream pathways such as protein kinase C (PKC) and mTOR signaling. Its involvement in neuronal development, synaptic plasticity, and T-cell receptor signaling underscores its physiological importance. Dysregulation of DGKe has been implicated in neurological disorders, immune dysfunctions, and cancer, making it a potential therapeutic target.
Recombinant DGKe proteins are typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) for structural and functional studies. These engineered proteins enable detailed biochemical characterization, including kinase activity assays, lipid-binding studies, and interaction mapping. Recent advances in cryo-EM and X-ray crystallography have begun to unravel DGKe’s conformational dynamics and substrate recognition mechanisms.
The development of DGKe-specific inhibitors or activators remains an active area of research, with implications for treating diseases linked to lipid signaling imbalances. Overall, DGKe recombinant proteins serve as essential tools for deciphering its biological roles and exploring therapeutic interventions.
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