| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGKz |
| Uniprot No | Q13574 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-928aa |
| 氨基酸序列 | MEPRDGSPEARSSDSESASASSSGSERDAGPEPDKAPRRLNKRRFPGLRLFGHRKAITKSGLQHLAPPPPTPGAPCSESERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVARMLKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPKEALEMYRKVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEDRDEGATDRLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGMDLTPKIQDLKPQCVVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVVLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRIQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLQQEPDGAGAKSPTCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTPRSLQGDAAPPQGEELIEAAKRNDFCKLQELHRAGGDLMHRDEQSRTLLHHAVSTGSKDVVRYLLDHAPPEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV |
| 预测分子量 | 103,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DGKζ(二酰基甘油激酶ζ亚型)重组蛋白研究的示例文献(注：文献为示例，实际引用需核实真实来源)：
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1. **文献名称**: *"Structural and functional characterization of human DGKζ recombinant protein in lipid signaling"*
**作者**: Smith, J.R., et al.
**摘要**: 本研究通过原核表达系统纯化了人源DGKζ重组蛋白，解析了其激酶结构域的三维结构，并验证了其对二酰基甘油(DAG)的催化活性。研究揭示了DGKζ在调节T细胞受体信号通路中的关键作用。
2. **文献名称**: *"DGKζ deficiency alters dendritic cell function via impaired mTORC1 signaling"*
**作者**: Tanaka, K., et al.
**摘要**: 通过重组DGKζ蛋白的体外功能实验，发现其缺失导致树突状细胞中mTORC1信号通路异常，进而影响免疫突触形成和抗原呈递效率，提示DGKζ在免疫调节中的潜在靶点价值。
3. **文献名称**: *"Recombinant DGKζ regulates prostate cancer cell invasion by modulating Rho GTPase activity"*
**作者**: Lee, H., & Wang, X.
**摘要**: 利用重组DGKζ蛋白进行细胞实验，证明其通过代谢DAG调控RhoA/ROCK通路活性，抑制前列腺癌细胞的迁移和侵袭，为靶向DGKζ的肿瘤治疗策略提供依据。
4. **文献名称**: *"Development of a high-yield mammalian expression system for DGKζ and its phosphoproteomics analysis"*
**作者**: Chen, L., et al.
**摘要**: 报道了一种哺乳动物细胞表达系统高效制备活性DGKζ重组蛋白的方法，结合磷酸化蛋白质组学，鉴定了多个与DGKζ相互作用并影响其催化功能的磷酸化位点。
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**注意**：以上文献为示例，实际研究中需通过PubMed、Web of Science等数据库检索真实文献(关键词如“DGKζ recombinant protein”“diacylglycerol kinase zeta”)。真实研究可能涉及DGKζ在神经退行性疾病、免疫代谢或癌症中的机制。
DGKζ (diacylglycerol kinase ζ) is a member of the diacylglycerol kinase (DGK) enzyme family, which plays a critical role in lipid signaling by catalyzing the conversion of diacylglycerol (DAG) to phosphatidic acid (PA). These lipid messengers regulate diverse cellular processes, including membrane trafficking, cytoskeletal reorganization, and nuclear signaling. Among DGK isoforms, DGKζ stands out due to its unique structural features, such as a nuclear localization signal and a myristoylated alanine-rich C-kinase substrate (MARCKS) homology domain, enabling its localization to both the cytoplasm and nucleus. It is particularly influential in modulating immune responses, neuronal development, and cancer progression by balancing DAG and PA levels.
The recombinant DGKζ protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper post-translational modifications. Its production typically involves gene cloning, transfection, and affinity purification (e.g., His-tag or GST-tag systems). Recombinant DGKζ retains enzymatic activity and structural integrity, making it a valuable tool for *in vitro* studies to dissect lipid metabolism pathways, screen kinase inhibitors, or investigate protein-lipid interactions.
Research on DGKζ has gained momentum due to its implications in diseases. For instance, in T-cell receptor signaling, DGKζ attenuates DAG-mediated activation of protein kinase C (PKC) and RasGRP, impacting immune tolerance and inflammation. Dysregulation of DGKζ is linked to cancer metastasis, neurodevelopmental disorders, and autoimmune conditions. Recombinant DGKζ facilitates mechanistic studies and drug discovery, offering potential therapeutic avenues. Its role in nuclear lipid signaling further underscores its importance in transcriptional regulation and stress responses, highlighting DGKζ as a multifaceted regulator in cellular homeostasis.
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