| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UCRP |
| Uniprot No | P05161 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-157aa |
| 氨基酸序列 | AWDLKVKMLGGNDFLVSVTNSMTVSELKKQIAQKIGVPAFQQRLAHQTAVLQDGLTLSSLGLGPSSTVMLVVQNCSEPLSILVRNERGHSNIYEVFLTQTVDTLKKKVSQREQVHEDQFWLSFEGRPMEDKELLGEYGLKPQCTVIKHLRLRGGGG |
| 预测分子量 | 21.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UCRP(泛素交叉反应蛋白)重组蛋白的3篇代表性文献摘要：
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1. **文献名称**：*"Cloning, Expression, and Functional Analysis of Ubiquitin Cross-Reactive Protein (UCRP) in Inflammatory Response"*
**作者**：Chen L, et al.
**摘要**：研究报道了人源UCRP基因在大肠杆菌中的重组表达与纯化，验证其通过调控ISG15修饰通路抑制炎症因子释放，为自身免疫疾病治疗提供潜在靶点。
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2. **文献名称**：*"Structural Insights into UCRP/UBL5: Implications for Ubiquitin-like Protein Folding"*
**作者**：Ryu MJ, Kim HT.
**摘要**：通过核磁共振解析重组UCRP的三维结构，发现其β-折叠构象对泛素样蛋白家族的功能保守性至关重要，为蛋白质工程改造提供依据。
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3. **文献名称**：*"High-Yield Production of Recombinant UCRP in Mammalian Cells and Its Role in Oxidative Stress Resistance"*
**作者**：Gupta S, et al.
**摘要**：利用HEK293细胞系统实现UCRP的高效分泌表达，实验证明重组蛋白通过激活Nrf2通路显著增强细胞对氧化应激的耐受性。
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*注：上述文献为示例性质，实际引用需以具体数据库(如PubMed、Web of Science)检索结果为准。建议补充关键词“UBL5”或“ISG15 pathway”扩大检索范围。*
**Background of UCRP Recombinant Protein**
UCRP (Ubiquitin Cross-Reactive Protein), also known as ISG15 (Interferon-Stimulated Gene 15), is a small ubiquitin-like protein involved in post-translational modification. It was first identified as a protein induced by type I interferons (IFNs) during immune responses, linking it to antiviral defense and inflammatory regulation. Structurally, UCRP shares homology with ubiquitin, featuring a conserved β-grasp fold, but it functions independently through a process called ISGylation—a covalent conjugation to target proteins via a cascade involving E1 (activating), E2 (conjugating), and E3 (ligating) enzymes.
UCRP plays dual roles: as a free molecule, it acts as a cytokine-like signaling molecule, modulating immune cell activity; as a conjugated form, it stabilizes or degrades target proteins, influencing pathways like protein degradation, DNA repair, and stress responses. Its expression is tightly regulated, spiking during viral infections, cancer, or autoimmune conditions.
Recombinant UCRP is engineered using expression systems (e.g., *E. coli* or mammalian cells) for research and therapeutic applications. It serves as a tool to study ISGylation mechanisms, host-pathogen interactions, and immune evasion strategies. Clinically, UCRP is explored as a biomarker for autoimmune diseases (e.g., lupus) and a potential target for antiviral or anticancer therapies. Its unique regulatory functions and interplay with ubiquitin pathways make it a critical subject in immunology and molecular biology research.
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