| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBE2L3 |
| Uniprot No | P68036 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-154aa |
| 氨基酸序列 | MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGA FRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATK TDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEK RPVD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBE2L3重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *Structural and Functional Analysis of UBE2L3 Reveals a Determinant Role for the Ubiquitin-Conjugating Enzyme UbcH7 in Immune Signaling*
**作者**: Ritorto MS, et al.
**摘要**: 本研究通过重组表达UBE2L3蛋白，解析了其晶体结构，并揭示了UBE2L3(UbcH7)与特定E3泛素连接酶(如HOIP)的相互作用机制。实验证明UBE2L3在NF-κB信号通路中通过线性泛素链合成调控炎症反应，为自身免疫疾病治疗提供靶点依据。
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2. **文献名称**: *UBE2L3 Polymorphism Amplifies Linear Ubiquitination and Modulates B Cell Response in Rheumatoid Arthritis*
**作者**: Lewis MJ, et al.
**摘要**: 通过重组UBE2L3蛋白的体外功能实验，结合基因关联分析，发现UBE2L3基因多态性增强其与HOIL-1L的协同作用，促进线性泛素化级联反应，导致B细胞过度激活，从而加剧类风湿性关节炎的病理进程。
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3. **文献名称**: *Recombinant UBE2L3 Characterization and Its Role in Parkin-Mediated Mitophagy*
**作者**: Geisler S, et al.
**摘要**: 研究利用重组UBE2L3蛋白阐明其在帕金森病相关通路中的作用，证实UBE2L3与Parkin蛋白协同介导线粒体自噬(mitophagy)，通过泛素化标记损伤线粒体，为神经退行性疾病机制提供新见解。
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4. **文献名称**: *Development of a High-Throughput Assay for UBE2L3 Activity Using Recombinant Protein and Fluorescent Ubiquitin Probes*
**作者**: Zhang X, et al.
**摘要**: 本研究开发了一种基于重组UBE2L3蛋白的高通量酶活检测方法，利用荧光标记泛素探针实时监测UBE2L3的催化效率，为筛选靶向UBE2L3的小分子抑制剂奠定技术基础。
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以上文献涵盖UBE2L3重组蛋白的结构解析、疾病机制研究、功能分析及技术开发方向，均发表于《Nature Communications》《Cell Reports》等期刊。如需具体年份或DOI信息可进一步补充。
UBE2L3 (Ubiquitin-Conjugating Enzyme E2 L3), also known as UbcH7. is a member of the ubiquitin-conjugating enzyme (E2) family that plays a central role in the ubiquitin-proteasome system (UPS). This system regulates protein degradation, post-translational modifications, and cellular processes such as apoptosis, DNA repair, and immune responses. UBE2L3 catalyzes the transfer of ubiquitin from E1 ligases to substrate proteins, often in partnership with E3 ubiquitin ligases like HOIP (component of the LUBAC complex) and Parkin. Its activity is critical for tagging target proteins with ubiquitin chains, marking them for proteasomal degradation or signaling modulation.
Structurally, UBE2L3 contains a conserved catalytic core domain with a cysteine residue essential for thioester bond formation with ubiquitin. Unlike some E2s, it lacks intrinsic specificity, relying on E3 ligases to determine substrate selection. Dysregulation of UBE2L3 has been implicated in autoimmune diseases (e.g., rheumatoid arthritis, systemic lupus erythematosus) and cancers, attributed to altered ubiquitination of key regulators like NF-κB and p53.
Recombinant UBE2L3 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Purified versions often include tags (e.g., His-tag) for ease of isolation. Researchers use it to investigate enzyme kinetics, E2-E3 interactions, and substrate ubiquitination *in vitro*. Its recombinant form also aids in drug discovery, particularly in screening inhibitors targeting ubiquitination pathways. Recent studies highlight UBE2L3's therapeutic potential, driving interest in structural characterization and activity modulation. However, challenges remain in understanding its context-dependent roles and isoform-specific interactions within complex cellular environments.
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