| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP1 |
| Uniprot No | O94782 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-785aa |
| 氨基酸序列 | MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKAS EYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLY FCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQ SLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDA QEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIE MDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQT RSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPS ILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPG NTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERR EDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVG EDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLS KINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKV TDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGG NTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRN SETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLF DDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL |
| 预测分子量 | 115 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于USP1重组蛋白的3篇参考文献概览：
1. **"USP1 deubiquitinase: cellular functions and therapeutic targeting in cancer"**
*作者：Huang et al.*
**摘要**：本研究综述了USP1在DNA损伤修复和干细胞维持中的作用，利用重组蛋白实验揭示了其通过去泛素化调控PCNA和FANCD2的机制，并探讨了USP1抑制剂在癌症治疗中的应用潜力。
2. **"Structural basis of ubiquitin recognition by USP1 for DNA translesion synthesis"**
*作者：Williams et al.*
**摘要**：通过解析USP1重组蛋白的晶体结构，阐明了其特异性识别泛素化PCNA的结构域，研究证实USP1通过去泛素化调控跨损伤DNA合成(TLS)的分子通路。
3. **"USP1 regulates cellular oxidative stress response through modulating Nrf2 stability"**
*作者：Li et al.*
**摘要**：利用体外表达的USP1重组蛋白，发现其通过去泛素化稳定转录因子Nrf2.从而增强细胞抗氧化应激能力，为USP1在氧化损伤相关疾病中的功能提供了新见解。
*注：以上为模拟文献案例，实际引用时建议通过PubMed等平台检索最新研究。*
USP1 (Ubiquitin-Specific Protease 1) is a deubiquitinating enzyme that plays a critical role in maintaining genomic stability by regulating DNA repair and replication processes. As a member of the ubiquitin-specific protease family, USP1 selectively removes ubiquitin moieties from target proteins, modulating their stability, localization, or activity. It is particularly known for its interaction with key DNA damage response (DDR) proteins, such as PCNA (Proliferating Cell Nuclear Antigen) and FANCD2 (Fanconi Anemia Group D2). USP1 deubiquitinates monoubiquitinated PCNA to terminate translesion DNA synthesis (TLS) and promotes the deubiquitination of FANCD2 to regulate the Fanconi anemia pathway, both essential for resolving replication stress and DNA crosslink repair.
Recombinant USP1 protein is engineered for in vitro studies to dissect its enzymatic mechanisms, substrate specificity, and regulatory interactions. Structurally, USP1 contains a catalytic domain responsible for its protease activity and a zinc-finger ubiquitin-binding domain. Notably, USP1 activity is tightly regulated through autocleavage and association with its co-factor UAF1 (USP1-Associated Factor 1), which stabilizes the enzyme and enhances its activity. Dysregulation of USP1 has been linked to cancer, as its overexpression in certain tumors may promote resistance to DNA-damaging therapies. Consequently, recombinant USP1 is widely used in drug discovery to screen for inhibitors that could sensitize cancer cells to chemotherapy or radiotherapy.
Research on recombinant USP1 also explores its role in stem cell maintenance and osteogenesis, highlighting its multifaceted biological significance. Its study provides insights into DDR pathways and potential therapeutic strategies targeting genomic instability-related diseases.
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