关键信息
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基因名
DND1
- 应用
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别名
RNA-binding motif. single-stranded-interacting protein 4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IYX4
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表达区间
1-353aa
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氨基酸序列
MQSKRDCELWCERVNPENKAALEAWVRETGIRLVQVNGQRKYGGPPPGWVGSPPPAGSEVFIGRLPQDVYEHQLIPLFQRVGRLYEFRLMMTFSGLNRGFAYARYSSRRGAQAAIATLHNHPLRPSCPLLVCRSTEKCELSVDGLPPNLTRSALLLALQPLGPGLQEARLLPSPGPAPGQIALLKFSSHRAAAMAKKALVEGQSHLCGEQVAVEWLKPDLKQRLRQQLVGPFLRSPQPEGSQLALARDKLGFQGARATLQLLCQRMKLGSPVFLTKCLGIGPAGWHRFWYQVVIPGHPVPFSGLIWVVLTLDGRDGHEVAKDAVSVRLLQALSESGANLLWSAGAEAGTMVKQ
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分子量
65.22 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
DND1 (Double strand RNA Binding Protein) is a vital component in the germ cell development and differentiation process in various organisms, including humans. Its key role lies in the regulation of gene expression associated with germline identity through RNA-binding mechanisms. Research into DND1 emerged from the need to understand the complexities of reproductive biology and the molecular pathways that underpin fertility and gametogenesis. Abnormalities in DND1 function have been linked to significant reproductive issues and various infertility disorders, making it a crucial target for therapeutic exploration. Advances in recombinant protein technology have facilitated the expression and purification of DND1, allowing researchers to investigate its structural properties and functional mechanisms in detail. By studying recombinant DND1 protein, scientists aim to elucidate its interactions with RNA and other cellular components, providing insights into its role in germ cell maintenance and development. This research is essential not only for understanding fundamental biological processes but also for developing potential treatments for reproductive health disorders linked to DND1 dysfunction. Overall, the study of recombinant DND1 protein stands at the intersection of molecular biology and reproductive medicine, paving the way for innovations in fertility treatments and our understanding of germline biology.
