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| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC123 |
| Uniprot No | O75794 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-336aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMKKEHVL HCQFSAWYPF FRGVTIKSVI LPLPQNVKDY LLDDGTLVVS GRDDPPTHSQ PDSDDEAEEI QWSDDENTAT LTAPEFPEFA TKVQEAINSL GGSVFPKLNW SAPRDAYWIA MNSSLKCKTL SDIFLLFKSS DFITRDFTQP FIHCTDDSPD PCIEYELVLR KWCELIPGAE FRCFVKENKL IGISQRDYTQ YYDHISKQKE EIRRCIQDFF KKHIQYKFLD EDFVFDIYRD SRGKVWLIDF NPFGEVTDSL LFTWEELISE NNLNGDFSEV DAQEQDSPAF RCTNSEVTVQ PSPYLSYRLP KDFVDLSTGE DAHKLIDFLK LKRNQQEDD |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDC123重组蛋白的3篇参考文献示例，包括文献名称、作者及摘要概述：
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1. **文献名称**: *"Expression and Functional Characterization of Recombinant CDC123 Protein in Eukaryotic Cells"*
**作者**: Zhang L, et al.
**摘要**: 本研究报道了CDC123重组蛋白在HEK293细胞中的表达与纯化，分析了其作为真核翻译起始因子eIF2γ亚基调节剂的功能。通过体外实验证实，重组CDC123能够促进eIF2复合体的组装，并增强应激条件下细胞的翻译恢复能力。
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2. **文献名称**: *"Structural Insights into CDC123 Role in Cell Cycle Regulation via X-ray Crystallography"*
**作者**: Patel RK, et al.
**摘要**: 利用重组CDC123蛋白的晶体结构解析，揭示了其与CDK1激酶的相互作用界面。研究阐明了CDC123通过构象变化调控细胞周期G2/M期转换的分子机制，为癌症治疗提供了潜在靶点。
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3. **文献名称**: *"Development of a Bacterial Expression System for High-Yield Production of Active CDC123"*
**作者**: Gupta S, et al.
**摘要**: 本文优化了在大肠杆菌中高效表达可溶性CDC123重组蛋白的工艺，通过密码子优化和温度调控提升产量。纯化后的蛋白具有ATP结合活性，为后续酶学研究和药物筛选提供了可靠工具。
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*注：以上文献为示例性质，实际引用需根据具体研究内容检索真实数据库(如PubMed、Google Scholar)获取。若需进一步协助定位真实文献，请提供更多研究背景或关键词。*
CDC123 recombinant protein, also known as cell division cycle protein 123. is a genetically engineered protein derived from the CDC123 gene. This protein plays a regulatory role in cellular processes, particularly in cell cycle progression and protein synthesis. Studies suggest that CDC123 functions as a scaffold or regulatory component in the assembly of multi-protein complexes, such as the eukaryotic translation initiation factor 2B (eIF2B), which is critical for initiating mRNA translation under stress conditions. Its involvement in these pathways links it to cellular stress responses, metabolic regulation, and cell proliferation.
The recombinant form of CDC123 is typically produced using expression systems like *E. coli* or mammalian cell cultures, enabling large-scale production for research and therapeutic applications. Purification methods often involve affinity tags (e.g., His-tag) to ensure high purity and stability. Researchers utilize CDC123 recombinant protein to investigate its molecular interactions, enzymatic activities, and structural features. Its role in eIF2B complex formation has drawn attention in studies related to neurodegenerative diseases, cancer, and metabolic disorders, where dysregulated protein synthesis or stress responses are implicated.
Additionally, CDC123 recombinant protein serves as a valuable tool for developing assays, antibodies, or inhibitors targeting pathways involved in cell cycle control or translational regulation. Its conserved sequence across species underscores its fundamental biological significance, making it a focus for both basic research and drug discovery efforts.
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