| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARSF |
| Uniprot No | P54793 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-590aa |
| 氨基酸序列 | HRVHDDKP NIVLIMVDDL GIGDLGCYGN DTMRTPHIDR LAREGVRLTQ HISAASLCSP SRSAFLTGRY PIRSGMVSSG NRRVIQNLAV PAGLPLNETT LAALLKKQGY STGLIGKWHQ GLNCDSRSDQ CHHPYNYGFD YYYGMPFTLV DSCWPDPSRN TELAFESQLW LCVQLVAIAI LTLTFGKLSG WVSVPWLLIF SMILFIFLLG YAWFSSHTSP LYWDCLLMRG HEITEQPMKA ERAGSIMVKE AISFLERHSK ETFLLFFSFL HVHTPLPTTD DFTGTSKHGL YGDNVEEMDS MVGKILDAID DFGLRNNTLV YFTSDHGGHL EARRGHAQLG GWNGIYKGGK GMGGWEGGIR VPGIVRWPGK VPAGRLIKEP TSLMDILPTV ASVSGGSLPQ DRVIDGRDLM PLLQGNVRHS EHEFLFHYCG SYLHAVRWIP KDDSGSVWKA HYVTPVFQPP ASGGCYVTSL CRCFGEQVTY HNPPLLFDLS RDPSESTPLT PATEPLHDFV IKKVANALKE HQETIVPVTY QLSELNQGRT WLKPCCGVFP FCLCDKEEEV SQPRGPNEKR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARSF重组蛋白的假设性参考文献示例(请注意，这些文献为示例性质，实际引用时请核实真实来源)：
1. **文献名称**: "Expression and Functional Characterization of Recombinant ArsF Protein in Escherichia coli"
**作者**: Smith J, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组ArsF蛋白，证实其具有结合砷酸盐的能力，并显著提升宿主细胞在砷污染环境中的存活率。
2. **文献名称**: "Crystal Structure Analysis of ArsF: Implications for Heavy Metal Detoxification"
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析了重组ArsF的三维结构，揭示了其独特的金属结合位点，为阐明砷抗性分子机制提供了结构基础。
3. **文献名称**: "ArsF-Mediated Arsenic Sequestration in Synthetic Biological Systems"
**作者**: Lee H, et al.
**摘要**: 利用重组ArsF蛋白构建人工生物膜系统，证明其可高效吸附环境中的砷污染物，拓展了其在生物修复技术中的应用潜力。
4. **文献名称**: "Heterologous Expression of ArsF in Arabidopsis Enhances Arsenic Tolerance"
**作者**: Wang L, et al.
**摘要**: 在拟南芥中异源表达重组ArsF蛋白，显著提高植物对砷毒性的耐受性，为开发抗砷作物提供了实验依据。
**建议**：实际研究中，请通过PubMed、Web of Science等数据库，以关键词“ARSF recombinant protein”或“ArsF + expression/function”检索最新文献，并注意确认“ARSF”在具体研究中的定义(如是否属于抗砷蛋白家族或其他类别)。
**Background of ARSF Recombinant Protein**
ARSF recombinant protein is a synthetic biomolecule engineered through advanced genetic recombination techniques, designed to serve specific functional or structural roles in biomedical research, therapeutic development, or industrial applications. The term "ARSF" typically denotes a fusion protein or a chimeric construct, where distinct protein domains or functional motifs are combined to enhance stability, activity, or targeting capabilities. Such proteins are often produced using recombinant DNA technology, involving the insertion of gene sequences into expression vectors (e.g., bacterial, yeast, or mammalian systems), followed by protein expression, purification, and characterization.
The development of ARSF proteins stems from the need to overcome limitations of natural proteins, such as short half-life, immunogenicity, or lack of specificity. By integrating domains like Fc regions for prolonged circulation, signal peptides for secretion, or affinity tags for simplified purification, ARSF proteins achieve tailored functionalities. For instance, fusion constructs may combine a therapeutic protein (e.g., a cytokine or enzyme) with a carrier molecule to improve pharmacokinetics.
ARSF recombinant proteins are pivotal in drug development, particularly for biologics such as monoclonal antibodies, enzyme replacements, or vaccines. They also serve as critical tools in basic research, enabling studies on protein-protein interactions, cellular signaling pathways, or disease mechanisms. Additionally, their applications extend to diagnostics, where engineered proteins act as detection probes or biosensors.
Despite their versatility, challenges remain, including ensuring proper folding, post-translational modifications, and scalability in production. Advances in synthetic biology, codon optimization, and expression host engineering continue to refine ARSF protein design, driving innovation in precision medicine and biotechnology.
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