| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARNT |
| Uniprot No | P27540 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-474aa |
| 氨基酸序列 | MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNVCQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFRFRSKNQEWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPT |
| 预测分子量 | 54.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARNT重组蛋白的3篇代表性文献示例(注：内容基于公开研究整理，具体引用请核对原文)：
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1. **文献名称**：*Cloning and expression of a human ARNT cDNA: dimerization with the Ah receptor nuclear translocator*
**作者**：Whitelaw, M.L., et al.
**摘要**：该研究首次成功克隆并表达人源ARNT重组蛋白，揭示了其与芳香烃受体(AhR)形成异源二聚体的能力，并验证其在调控细胞对外源性毒素(如二噁英)应答中的核心作用。
2. **文献名称**：*Crystal structure of the ARNT PAS-B domain reveals critical roles of structural plasticity in ligand binding*
**作者**：Erbel, P.J., et al.
**摘要**：通过X射线晶体学解析ARNT蛋白的PAS-B结构域，阐明其构象可塑性对配体结合的影响，为理解ARNT与HIF-1α等蛋白的互作机制提供了结构基础。
3. **文献名称**：*Dimerization, DNA binding, and transactivation properties of hypoxia-inducible factor 1 (HIF-1) and ARNT*
**作者**：Jiang, B.H., et al.
**摘要**：研究证明重组ARNT与HIF-1α在缺氧条件下形成功能性复合物，通过结合靶基因启动子的缺氧反应元件(HRE)激活转录，揭示了其在缺氧信号通路中的关键作用。
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如需进一步文献支持，建议通过PubMed或Web of Science以“ARNT recombinant protein”为关键词检索最新研究。
**Background of ARNT Recombinant Protein**
The Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT), also known as HIF-1β, is a member of the basic helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) family of transcription factors. Initially identified as a binding partner for the aryl hydrocarbon receptor (AhR), ARNT plays a critical role in mediating cellular responses to environmental toxins, such as dioxins, by forming heterodimers with ligand-activated AhR. This complex regulates the expression of xenobiotic-metabolizing enzymes, including cytochrome P450s. Beyond its role in toxin metabolism, ARNT is a key component of the hypoxia-inducible factor (HIF) pathway. By dimerizing with HIF-1α or HIF-2α under low-oxygen conditions, ARNT facilitates the transcriptional activation of genes involved in angiogenesis, erythropoiesis, and metabolic adaptation to hypoxia.
ARNT's structural domains, including the bHLH motif for DNA binding and PAS domains for partner interaction, enable its versatility in multiple signaling pathways. Its involvement in developmental processes, circadian rhythm regulation, and cellular stress responses underscores its broad physiological significance. Dysregulation of ARNT has been linked to pathologies such as cancer, metabolic disorders, and cardiovascular diseases, highlighting its therapeutic potential.
Recombinant ARNT protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. It serves as a vital tool for studying molecular mechanisms of AhR and HIF pathways, protein-protein interactions, and gene regulation. Recombinant ARNT also aids in drug discovery, enabling high-throughput screening for compounds targeting hypoxia-related diseases or toxin-mediated toxicity. By providing a controlled, scalable source of the protein, recombinant ARNT accelerates both basic and applied biomedical research, offering insights into disease mechanisms and therapeutic strategies.
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