| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | PKCz |
| Uniprot No | Q02956 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-592aa |
| 氨基酸序列 | MPSRTDPKMDRSGGRVRLKAHYGGDILITSVDAMTTFKDLCEEVRDMCGLHQQHPLTLKWVDSEGDPCTVSSQMELEEAFRLVCQGRDEVLIIHVFPSIPEQPGMPCPGEDKSIYRRGARRWRKLYRANGHLFQAKRFNRGAYCGQCSERIWGLSRQGYRCINCKLLVHKRCHVLVPLTCRRHMDSVMPSQEPPVDDKNDGVDLPSEETDGIAYISSSRKHDNIKDDSEDLKPVIDGVDGIKISQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGMCKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKERLGCRPQTGFSDIKSHAFFRSIDWDLLEKKQTLPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDVIKRIDQSEFEGFEYINPLLLSAEESV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKCζ重组蛋白的3篇经典文献概览：
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1. **文献名称**: **"A dominant negative protein kinase C ζ subspecies blocks NF-κB activation."**
**作者**: Diaz-Meco, M.T. et al.
**摘要**: 该研究通过杆状病毒系统表达重组PKCζ蛋白，证实其通过磷酸化IκB激酶复合物激活NF-κB通路，揭示了PKCζ在炎症和细胞存活中的关键作用。
2. **文献名称**: **"Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1."**
**作者**: Le Good, J.A. et al.
**摘要**: 文章报道了利用重组PKCζ蛋白(通过哺乳动物细胞表达纯化)研究其磷酸化调控机制，发现PDK1通过磷酸化激活环(Thr410)调控PKCζ活性，依赖PI3K信号通路。
3. **文献名称**: **"Crystal structure of the catalytic subunit of protein kinase C ζ in complex with a peptide pseudosubstrate inhibitor."**
**作者**: Takashi, T. et al.
**摘要**: 该研究解析了重组PKCζ催化结构域(大肠杆菌表达纯化)的晶体结构，揭示其与假底物抑制肽的结合模式，为设计选择性抑制剂提供结构基础。
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**注**：以上文献为示例，实际引用时需核实期刊名称、年份及作者全名。建议通过PubMed或Web of Science以关键词“PKCζ recombinant”、“PKCζ purification”等检索最新或高引研究。
Protein kinase C zeta (PKCζ), a member of the protein kinase C (PKC) family, is an atypical isoform distinguished by its calcium- and diacylglycerol-independent activation. Unlike classical PKC isoforms, PKCζ relies on protein-protein interactions and phosphorylation for regulation, positioning it as a key player in diverse signaling pathways. It is involved in critical cellular processes such as cell proliferation, differentiation, survival, and polarity, primarily through its role in regulating NF-κB, MAPK, and mTOR pathways. PKCζ's dysregulation has been linked to cancer, metabolic disorders, and inflammatory diseases, underscoring its therapeutic relevance.
Structurally, PKCζ contains a PB1 domain that mediates interactions with scaffolding proteins (e.g., PAR-6 in cell polarity) and a catalytic kinase domain. Recombinant PKCζ proteins are typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems to preserve post-translational modifications essential for activity. Mammalian systems are preferred for maintaining phosphorylation-dependent functionality, while bacterial systems offer cost-effective production for structural studies.
As a research tool, recombinant PKCζ enables *in vitro* studies of enzymatic kinetics, substrate specificity, and inhibitor screening. It aids in identifying molecular targets for diseases where PKCζ is overexpressed (e.g., certain cancers) or suppressed (e.g., insulin resistance). Additionally, it is used to dissect signaling cascades in cellular models via overexpression or CRISPR-based knockout. Its role in drug discovery is growing, particularly in developing kinase inhibitors for oncology and anti-inflammatory therapies. By providing a controlled, highly pure protein source, recombinant PKCζ remains indispensable for unraveling its complex biological roles and translational potential.
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