| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKCh |
| Uniprot No | P24723 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-683aa |
| 氨基酸序列 | MSSGTMKFNG YLRVRIGEAV GLQPTRWSLR HSLFKKGHQL LDPYLTVSVD QVRVGQTSTK QKTNKPTYNE EFCANVTDGG HLELAVFHET PLGYDHFVAN CTLQFQELLR TTGASDTFEG WVDLEPEGKV FVVITLTGSF TEATLQRDRI FKHFTRKRQR AMRRRVHQIN GHKFMATYLR QPTYCSHCRE FIWGVFGKQG YQCQVCTCVV HKRCHHLIVT ACTCQNNINK VDSKIAEQRF GINIPHKFSI HNYKVPTFCD HCGSLLWGIM RQGLQCKICK MNVHIRCQAN VAPNCGVNAV ELAKTLAGMG LQPGNISPTS KLVSRSTLRR QGKESSKEGN GIGVNSSNRL GIDNFEFIRV LGKGSFGKVM LARVKETGDL YAVKVLKKDV ILQDDDVECT MTEKRILSLA RNHPFLTQLF CCFQTPDRLF FVMEFVNGGD LMFHIQKSRR FDEARARFYA AEIISALMFL HDKGIIYRDL KLDNVLLDHE GHCKLADFGM CKEGICNGVT TATFCGTPDY IAPEILQEML YGPAVDWWAM GVLLYEMLCG HAPFEAENED DLFEAILNDE VVYPTWLHED ATGILKSFMT KNPTMRLGSL TQGGEHAILR HPFFKEIDWA QLNHRQIEPP FRPRIKSRED VSNFDPDFIK EEPVLTPIDE GHLPMINQDE FRNFSYVSPE LQP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKCη(Protein Kinase C eta型)重组蛋白的3篇参考文献示例(内容基于典型研究方向模拟，具体文献需以实际检索为准)：
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1. **标题**：*Cloning and Functional Characterization of Human Protein Kinase C eta (PKCη) Expressed in Baculovirus-Infected Insect Cells*
**作者**：Y. Takai, M. Kishimoto, et al.
**摘要**：本研究成功克隆了人源PKCη基因，并在杆状病毒-昆虫细胞表达系统中实现重组蛋白的高效表达。通过亲和层析纯化后，验证了其激酶活性依赖于磷脂酰丝氨酸和二酰基甘油的协同激活，为后续信号通路研究提供工具。
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2. **标题**：*Recombinant PKCη as a Therapeutic Target in Skin Cancer: Role in Apoptosis Regulation*
**作者**：A. Newton, C. Brognard
**摘要**：文章利用重组PKCη蛋白探究其在皮肤癌细胞凋亡中的作用。实验表明，PKCη过表达通过抑制促凋亡蛋白活性增强细胞存活，提示其作为癌症治疗靶点的潜力。
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3. **标题**：*Optimized Expression and Purification of Active PKCη in E. coli Using Chaperone Co-Expression*
**作者**：S. Tanaka, H. Shimizu
**摘要**：作者优化了大肠杆菌中PKCη的重组表达策略，通过共表达分子伴侣蛋白解决包涵体问题，获得可溶性活性蛋白。纯化后的蛋白适用于体外激酶抑制剂高通量筛选。
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**备注**：若需具体文献，建议在PubMed或Web of Science中以关键词“PKCη recombinant”、“Protein Kinase C eta expression”检索，并筛选近十年高被引研究。
PKCh (Protein Kinase C eta) is a member of the Protein Kinase C (PKC) family, a group of serine/threonine kinases critical in regulating diverse cellular processes, including proliferation, differentiation, apoptosis, and immune responses. Discovered in the 1990s, PKCh is categorized under the "novel" PKC subfamily (nPKC), characterized by calcium-independent activation but dependence on diacylglycerol (DAG) and phospholipids. Unlike classical PKCs, PKCh lacks the C2 domain responsible for calcium binding, enabling unique regulatory mechanisms. It is predominantly expressed in epithelial tissues, immune cells, and the central nervous system, where it modulates signaling pathways linked to inflammation, skin barrier function, and neuronal survival.
Recombinant PKCh protein is engineered using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to produce functional kinase for research. Its recombinant form retains enzymatic activity, allowing studies on substrate specificity, inhibitor screening, and structural analysis. Researchers utilize PKCh recombinant proteins to investigate its role in diseases such as cancer (e.g., promoting tumor cell survival), autoimmune disorders (e.g., regulating T-cell activation), and neurodegenerative conditions. Additionally, it serves as a tool to dissect PKC signaling crosstalk with pathways like MAPK/ERK and NF-κB. Despite progress, challenges remain in understanding tissue-specific functions and developing isoform-selective inhibitors, underscoring the importance of recombinant PKCh in both basic and therapeutic research.
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