| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKD3 |
| Uniprot No | O94806 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-890aa |
| 氨基酸序列 | MSANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSADEIHEGDLVEVVLSALATVEDFQIRPHTLYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPEYVALPSEESHVHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYTRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPSSLGTDTDIPMDIDNNDINSDSSRGLDDTEEPSPPEDKMFFLDPSDLDVERDEEAVKTISPSTSNNIPLMRVVQSIKHTKRKSSTMVKEGWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSEILRISSPRDFTNISQGSNPHCFEIITDTMVYFVGENNGDSSHNPVLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLSTSISVSNCQIQENVDISTVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPWREISGEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHESDDARWEIHAYTHNLVYPKHFIMAPNPDDMEEDP |
| 预测分子量 | 100,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKD3重组蛋白的模拟参考文献(内容为虚构，仅供参考)：
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1. **文献名称**: *Expression and purification of recombinant human PKD3 in Escherichia coli for structural studies*
**作者**: Zhang, L., et al.
**摘要**: 该研究报道了利用大肠杆菌表达系统高效表达重组人PKD3蛋白，并通过镍柱亲和层析和尺寸排阻色谱纯化，获得高纯度蛋白。纯化的PKD3成功用于X射线晶体学分析，揭示了其激酶结构域的关键构象特征。
2. **文献名称**: *Functional characterization of PKD3 in cancer cell migration using a mammalian recombinant expression system*
**作者**: Gupta, R., & Lee, S.
**摘要**: 作者构建了哺乳动物细胞(HEK293)表达的重组PKD3蛋白，验证其磷酸化活性及对下游信号通路(如MAPK)的调控作用。实验表明，PKD3过表达显著增强乳腺癌细胞的迁移能力，提示其作为潜在治疗靶点。
3. **文献名称**: *Development of a kinase activity assay for recombinant PKD3 and screening of small-molecule inhibitors*
**作者**: Müller, T., et al.
**摘要**: 研究建立了基于荧光共振能量转移(FRET)的PKD3激酶活性检测体系，利用重组PKD3蛋白筛选出多个特异性小分子抑制剂，并验证其在前列腺癌细胞中的抗增殖效果。
4. **文献名称**: *Role of PKD3 in lipid metabolism: Insights from recombinant protein-mediated in vitro assays*
**作者**: Park, J., et al.
**摘要**: 通过表达纯化的重组PKD3蛋白，研究发现其与脂代谢相关蛋白(如AMPK)的相互作用，并证明PKD3通过磷酸化调控脂滴形成，为代谢性疾病机制提供了新视角。
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**注**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
PKD3 (Protein Kinase D3), also known as PRKD3. is a serine/threonine kinase belonging to the PKD family, which includes PKD1 and PKD2. It is part of the calcium/calmodulin-dependent protein kinase superfamily and shares structural features such as a catalytic domain, a pleckstrin homology domain, and a cysteine-rich regulatory domain. PKD3 is activated by diacylglycerol (DAG) and protein kinase C (PKC) signaling, playing roles in diverse cellular processes, including proliferation, migration, apoptosis, and membrane trafficking. Unlike PKD1 and PKD2. PKD3 exhibits distinct tissue expression patterns and functional specificity, with elevated levels observed in certain cancers, such as breast and prostate cancer, suggesting its involvement in tumor progression and metastasis.
Recombinant PKD3 proteins are engineered using expression systems like *E. coli*, insect, or mammalian cells to produce functional, purified forms of the kinase for research. These proteins often include affinity tags (e.g., His-tag, GST) for simplified purification. Recombinant PKD3 enables *in vitro* studies of its enzymatic activity, substrate interactions, and regulatory mechanisms. It is critical for structural analyses, inhibitor screening, and understanding PKD3's role in diseases. Research highlights its potential as a therapeutic target, particularly in oncology, where aberrant PKD3 signaling contributes to drug resistance and metastatic behavior. By providing a standardized, active form of the kinase, recombinant PKD3 accelerates mechanistic studies and drug discovery efforts aimed at modulating its activity in pathological contexts.
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