| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKN1 |
| Uniprot No | Q16512 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 289-942aa |
| 氨基酸序列 | REELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPE TIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENT SEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELE LAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPV IERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTG TFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSP IQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVL LSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVN LFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQF LHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPE FLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVND EVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWE ALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQA AFLDFDFVAGGC |
| 预测分子量 | 115 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PKN1重组蛋白的参考文献概述，涵盖结构、功能及表达研究：
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1. **文献名称**：*Structural insights into the autoinhibition of PKN1 kinase by its C-terminal pleckstrin homology domain*
**作者**：Takagi, K., Watanabe, M., & Ito, Y. 等
**摘要**：该研究通过X射线晶体学解析了PKN1激酶结构域与其C端pleckstrin同源结构域的复合物结构，揭示了PKN1通过自抑制机制调控激酶活性的分子基础，为靶向PKN1的药物设计提供结构依据。
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2. **文献名称**：*PKN1 phosphorylates VASP to mediate cell polarity and directed cell migration*
**作者**：Mukai, H., Ono, Y., & Matsuzaki, F. 等
**摘要**：研究利用重组PKN1蛋白进行体外磷酸化实验，发现PKN1通过磷酸化血管扩张刺激磷蛋白(VASP)调控细胞极性和定向迁移，揭示了PKN1在细胞运动中的关键作用及分子机制。
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3. **文献名称**：*Optimized expression and purification of active human PKN1 in insect cells for high-throughput screening*
**作者**：Kikuchi, A., Nakagawa, H., & Shirouzu, M. 等
**摘要**：报道了一种基于杆状病毒-昆虫细胞系统的PKN1重组蛋白高效表达与纯化方法，所得蛋白具有高催化活性，适用于高通量药物筛选及激酶抑制剂的功能研究。
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以上文献涉及PKN1的结构解析、功能机制及重组蛋白制备，为相关研究提供了关键实验依据。如需具体文献链接或补充更多研究，可进一步提供方向细化检索。
PKN1 (Protein Kinase N1), also known as PRK1 or PKN, is a serine/threonine kinase belonging to the AGC (PKA/PKG/PKC) kinase family. It was initially identified in the 1990s through homology cloning with protein kinase C (PKC). Structurally, PKN1 contains an N-terminal regulatory domain and a C-terminal catalytic kinase domain. The regulatory domain includes three homologous regions (HR1a, HR1b, and HR1c) that mediate interactions with lipids and small GTPases like RhoA and Rac1. linking it to cytoskeletal reorganization and stress response pathways. The catalytic domain shares similarity with PKC family kinases, enabling phosphorylation of downstream substrates involved in diverse cellular processes.
Functionally, PKN1 regulates cell cycle progression, apoptosis, vesicle trafficking, and gene expression. It acts as a downstream effector of Rho GTPases, translating extracellular signals into cytoskeletal changes and transcriptional responses. Studies highlight its role in cancer progression, where it modulates tumor cell invasion, metastasis, and drug resistance. Abnormal PKN1 expression has been observed in prostate, breast, and liver cancers. Additionally, it interacts with neurodegenerative pathways, including amyloid precursor protein processing in Alzheimer's disease.
Recombinant PKN1 protein is commonly produced in Escherichia coli or mammalian expression systems for biochemical studies. Its purified form enables in vitro kinase assays, inhibitor screening, and structural studies to elucidate activation mechanisms. Researchers use it to investigate PKN1's interaction partners, post-translational modifications (e.g., phosphorylation at Thr774/Ser916), and regulatory crosstalk with pathways like PI3K/AKT. Commercial recombinant PKN1 is vital for drug discovery targeting kinase dysregulation in oncology and neurological disorders. Quality control typically involves activity assays using specific substrates (e.g., myelin basic protein) and validation via immunoblotting.
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