| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKN2 |
| Uniprot No | Q16513 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-984aa |
| 氨基酸序列 | MASNPERGEILLTELQGDSRSLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKELKIKEGAENLRKVTTDKKSLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDITDCPRTPDTPNNDPRCSTSNNRLKALQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELRMEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLNEVPKNHPKSRIIIEELSLVAASPTLSPRQSMISTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSKATSVALPGWSPSETRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTSWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQAPVPTTVPVVDVRIPQLAPPASDSTVTKLDFDLEPEPPPAPPRASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEYKPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDVKKHPFFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQEMFRDFDYIADWC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKN2重组蛋白的参考文献示例(注意：部分文献信息为假设性概括，实际引用时请核实准确性)：
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1. **Takahashi, K. et al. (2003)**
*"Recombinant expression and characterization of human protein kinase N2: Regulation by Rho GTPases"*
**摘要**：本研究报道了人源PKN2激酶结构域的重组表达与纯化，利用昆虫细胞系统成功获得活性蛋白，并证明其激酶活性受Rho家族GTP酶(如RhoA)的直接调控，为后续功能研究奠定基础。
2. **Vincent, S. & Settleman, J. (2007)**
*"PKN2 modulates cytoskeletal dynamics via Rho signaling in cancer cell invasion"*
**摘要**：通过重组PKN2蛋白的体外磷酸化实验，作者发现PKN2与Rho效应蛋白的相互作用可调节肌动蛋白重构，揭示其在肿瘤细胞迁移和侵袭中的关键作用。
3. **Lee, J. et al. (2012)**
*"Crystal structure of the PKN2 kinase domain reveals autoinhibitory mechanisms"*
**摘要**：本研究解析了重组PKN2激酶结构域的晶体结构，发现其C端调控域通过自抑制构象限制激酶活性，为设计靶向PKN2的小分子抑制剂提供结构依据。
4. **Chen, L. et al. (2018)**
*"High-throughput screening identifies novel PKN2 inhibitors using recombinant kinase assays"*
**摘要**：作者通过大肠杆菌表达系统获得高纯度重组PKN2蛋白，并建立高通量筛选平台，成功鉴定出多个选择性抑制PKN2的小分子化合物，具有潜在抗肿瘤应用价值。
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**注意**：以上文献信息为示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献，结合关键词如“PKN2 recombinant protein”“PKN2 kinase activity”等获取准确信息。
**Background of PKN2 Recombinant Protein**
PKN2 (Protein Kinase N2), also known as PRKCL2. is a serine/threonine kinase belonging to the protein kinase C (PKC) superfamily. It plays a critical role in regulating diverse cellular processes, including cytoskeletal organization, cell proliferation, apoptosis, and migration. Structurally, PKN2 contains an N-terminal regulatory region with a conserved C2-like domain and three antiparallel coiled-coil motifs, as well as a C-terminal catalytic kinase domain. The N-terminal region mediates interactions with small GTPases like RhoA and Rac1. linking PKN2 to Rho-mediated signaling pathways involved in cell adhesion and motility.
Recombinant PKN2 protein is engineered using expression systems such as *E. coli* or mammalian cells, often fused with tags (e.g., GST, His) for purification and detection. This allows researchers to study its enzymatic activity, substrate specificity, and interactions in vitro. PKN2 is implicated in cancer progression, as it regulates pathways like mTOR and NF-κB, influencing tumor cell survival and invasion. Additionally, PKN2 interacts with components of the Wnt/β-catenin pathway, suggesting roles in development and tissue homeostasis.
Studies using PKN2 recombinant protein have advanced understanding of its phosphorylation targets, including proteins like β-catenin and MYPT1. and its modulation by lipids or kinases like PDK1. Despite its importance, PKN2’s functional overlap with other PKN family members (PKN1 and PKN3) complicates mechanistic studies, necessitating isoform-specific tools. Recombinant PKN2 remains a vital reagent for biochemical assays, inhibitor screening, and structural studies, with potential therapeutic applications in cancer and fibrosis. Ongoing research aims to clarify its context-dependent roles and validate its candidacy as a drug target.
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