| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKR |
| Uniprot No | P30613 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-574aa |
| 氨基酸序列 | MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS |
| 预测分子量 | 63.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKR重组蛋白的3篇参考文献及其摘要概括：
1. **《Production of Recombinant Human PKR in Escherichia coli: Purification and Characterization》**
*作者：Williams BR et al.*
摘要：该研究报道了在大肠杆菌中高效表达重组人源PKR蛋白的方法，通过亲和层析纯化获得高纯度蛋白，并验证了其激酶活性及双链RNA依赖的自磷酸化特性。
2. **《Structural and Functional Analysis of PKR Kinase Domain by Recombinant Protein Expression》**
*作者：Nanduri S et al.*
摘要：作者通过昆虫细胞系统表达重组PKR激酶结构域，结合X射线晶体学分析其三维结构，揭示了PKR与ATP结合的关键位点及激活机制。
3. **《Recombinant PKR as a Tool for Antiviral Drug Screening》**
*作者：García MA et al.*
摘要：研究利用哺乳动物细胞表达体系制备功能性重组PKR，开发了一种基于PKR活性抑制的高通量筛选平台，用于鉴定靶向PKR的抗病毒候选化合物。
注：以上文献名称与作者为示例性简化内容，实际文献需通过PubMed或学术数据库检索确认准确信息。
Protein kinase R (PKR), also known as eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2), is an interferon-induced serine/threonine kinase that plays a critical role in innate immunity and cellular stress responses. Discovered in the context of viral infection studies, PKR is activated by double-stranded RNA (dsRNA), a molecular signature of many viruses. This activation triggers a cascade of antiviral mechanisms, primarily through phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2α), which halts global protein synthesis to limit viral replication. Beyond its antiviral role, PKR regulates pathways linked to apoptosis, inflammation, and cellular homeostasis, implicating it in diverse pathologies, including cancer, neurodegenerative diseases, and metabolic disorders.
Recombinant PKR proteins are engineered to study its structure-function relationships, signaling mechanisms, and therapeutic potential. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), recombinant PKR retains its dsRNA-binding domains and catalytic activity. Researchers utilize these proteins to dissect its interaction with viral RNAs, host proteins, and small-molecule inhibitors. Structural studies using recombinant PKR have revealed its autoinhibitory dimeric conformation and activation dynamics. Additionally, recombinant PKR serves as a tool for drug screening, aiming to modulate its activity in diseases where dysregulated PKR contributes to pathogenesis. Its dual role as a pathogen sensor and a regulator of cell fate underscores its importance in both basic research and translational applications.
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