| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKIg |
| Uniprot No | O70139 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-76aa |
| 氨基酸序列 | MMEVESSYSD FISCDRTGRR NAVPDIQGDS EAVSVRKLAG DMGELALEGA EGQAEGSTPD KEASSQPESS DANTSS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKIg(蛋白激酶抑制因子G)重组蛋白的3篇模拟参考文献示例，涵盖表达、功能及结构研究：
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1. **文献名称**：*"High-yield production of recombinant PKIg in Escherichia coli: Purification and characterization for kinase inhibition assays"*
**作者**：Smith, J.R., et al.
**摘要**：本研究成功克隆了人类PKIg基因，并利用大肠杆菌表达系统实现高效重组表达。通过镍柱亲和层析纯化获得高纯度蛋白，体外活性实验证实其有效抑制cAMP依赖性蛋白激酶(PKA)活性，为后续药理学研究提供工具。
2. **文献名称**：*"Structural insights into PKIg-mediated inhibition of PKA via X-ray crystallography"*
**作者**：Chen, L., et al.
**摘要**：通过解析重组PKIg与PKA催化亚基的复合物晶体结构，揭示了PKIg通过N端结构域与ATP结合位点竞争性结合，阻断PKA底物磷酸化的分子机制，为设计新型激酶抑制剂奠定结构基础。
3. **文献名称**：*"Functional modulation of PKIg in neuronal cAMP signaling pathways using recombinant protein delivery"*
**作者**：Johnson, M.T., et al.
**摘要**：利用重组PKIg蛋白在神经元细胞中特异性抑制PKA活性，证实其调控突触可塑性和CREB信号通路的关键作用，提示PKIg可能作为神经退行性疾病治疗的潜在靶点。
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**注**：以上文献为示例，实际研究中建议通过数据库(如PubMed、Google Scholar)以“PKIg recombinant”、“Protein Kinase Inhibitor Gamma expression”等关键词检索最新成果。若需具体文献，请提供更多背景信息以便精准定位。
**Background of PKIγ Recombinant Protein**
Protein kinase inhibitor gamma (PKIγ) is a member of the PKI family, which regulates the activity of protein kinase A (PKA), a key enzyme in the cAMP signaling pathway. PKA plays a central role in cellular processes such as metabolism, gene expression, and apoptosis. PKIγ specifically binds to the catalytic subunit of PKA, inhibiting its kinase activity and modulating downstream signaling. This regulation is critical for maintaining cellular homeostasis and responding to extracellular stimuli.
Recombinant PKIγ protein is engineered using molecular cloning techniques, where the PKIγ gene is expressed in heterologous systems like *E. coli* or mammalian cell lines. The recombinant protein is purified to high homogeneity, often tagged with affinity markers (e.g., His-tag) for efficient isolation. Its production enables precise study of PKA-PKI interactions, structural analysis, and screening of therapeutic agents targeting cAMP-dependent pathways.
Research on PKIγ has expanded due to its potential roles in diseases, including cancer and cardiovascular disorders. Dysregulation of PKA signaling is linked to tumor progression, and PKIγ-based tools may help elucidate mechanisms or develop inhibitors. Additionally, recombinant PKIγ serves as a critical reagent in *in vitro* kinase assays, providing insights into signal transduction networks.
The development of PKIγ recombinant protein underscores advancements in protein engineering, offering a reproducible and scalable source for functional studies. Its application bridges basic research and drug discovery, highlighting its importance in both academic and pharmaceutical contexts.
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