| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD9 |
| Uniprot No | P21926 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 112-195aa |
| 氨基酸序列 | SHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQ FISDICPKKDVLETFTVKSCPDAIKEVFDNKFHI |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD9重组蛋白的3篇代表性文献及其摘要(内容基于领域内典型研究方向整合而成)：
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1. **标题**：*Structural characterization of recombinant CD9 tetraspanin: Insights into membrane protein architecture*
**作者**：Higuchi, T. et al.
**摘要**：该研究通过杆状病毒-昆虫细胞系统成功表达并纯化CD9重组蛋白，利用冷冻电镜技术解析其跨膜结构域的三维构象，揭示了CD9与其他四跨膜蛋白相互作用的潜在分子机制，为研究其在细胞膜微域中的功能提供结构基础。
2. **标题**：*CD9-recombinant protein modulates integrin-dependent cell adhesion and metastasis*
**作者**：Boucheix, C. et al.
**摘要**：研究团队通过哺乳动物细胞表达CD9重组蛋白，发现其通过结合整合素β1调控肿瘤细胞黏附和迁移能力。实验表明，重组CD9可抑制黑色素瘤细胞的侵袭性，提示其在癌症治疗中的潜在应用价值。
3. **标题**：*Recombinant CD9 in extracellular vesicles: Role in intercellular communication*
**作者**：Raposo, G. et al.
**摘要**：本研究利用重组CD9蛋白标记外泌体，证实CD9通过参与囊泡膜融合过程促进细胞间物质传递。实验进一步表明，CD9缺失会显著降低外泌体对受体细胞的信号调控能力，凸显其在细胞通讯中的关键作用。
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**注**：以上文献为示例性整合内容，实际引用时建议通过PubMed或Web of Science等平台检索最新文献。如需具体文章信息，可提供研究方向进一步筛选。
CD9 recombinant protein is derived from the CD9 gene, which encodes a member of the tetraspanin family—a group of cell-surface proteins characterized by four transmembrane domains. CD9 plays critical roles in cellular processes such as adhesion, migration, signal transduction, and membrane fusion. It interacts with integrins, growth factors, and other tetraspanins to modulate cellular communication, particularly in immune responses, fertilization, and cancer progression. Notably, CD9 is implicated in exosome biogenesis, influencing intercellular signaling and disease pathways.
The recombinant form of CD9 is produced using engineered expression systems (e.g., mammalian, insect, or bacterial cells) to ensure high purity and bioactivity. Researchers utilize CD9 recombinant protein to study its molecular interactions, regulatory mechanisms, and therapeutic potential. For example, it has been applied in cancer research to explore its dual role as both a metastasis suppressor (by inhibiting cell motility) and a promoter of tumor cell invasion (via exosome-mediated signaling). In immunology, CD9’s involvement in T-cell activation and antigen presentation is a key focus. Additionally, studies on viral infections (e.g., HIV) investigate CD9’s ability to facilitate viral entry or modulate host cell responses.
Quality control for CD9 recombinant protein includes validation via Western blot, ELISA, and functional assays (e.g., cell adhesion or fusion tests). Its applications span in vitro binding studies, antibody development, and as a reference standard in diagnostics. By bypassing the complexity of native membrane-bound CD9. the recombinant form offers a versatile tool for dissecting its biological roles and advancing drug discovery.
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